Nanoplastics (NPs) affect fertility. We evaluated the effects of NPs treatment on luteal and endothelial cells. We examined crucial markers of growth and redox status. NPs treatment did not induce changes in ATP levels in luteal cells, while it increased (p< 0.05) their proliferation. In endothelial cells, no change in proliferation was detected, while an increase (p<0.05) in ATP levels was observed. The increase of reactive oxygen species, superoxide anion (p<0.05) and nitric oxide (p<0.001) was detected in both cell types, which also showed changes in superoxide dismutase enzyme activity as well as an increase of non-enzymatic antioxidant power (p<0.05). A decrease (p<0.05) in progesterone production as well as an increase of vascular endothelial growth factor A levels were detected (p<0.05). In addition, a dose-dependent accumulation of NPs in endothelial cells was shown, that likely occurred through adhesion and internalization. Results underline potential risk of NPs for corpus luteum functionality.
The porcine corpus luteum as a model for studying the effects of nanoplastics / Basini, Giuseppina; Bertini, Simone; Bussolati, Simona; Zappavigna, Francesca; Berni, Melissa; Scaltriti, Erika; Ramoni, Roberto; Grolli, Stefano; Quintavalla, Fausto; Grasselli, Francesca. - In: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY. - ISSN 1382-6689. - 110:(2024). [10.1016/j.etap.2024.104503]
The porcine corpus luteum as a model for studying the effects of nanoplastics
Giuseppina Basini
;Simone Bertini;Simona Bussolati;Erika Scaltriti;Roberto Ramoni;Stefano Grolli;Fausto Quintavalla;Francesca Grasselli
2024-01-01
Abstract
Nanoplastics (NPs) affect fertility. We evaluated the effects of NPs treatment on luteal and endothelial cells. We examined crucial markers of growth and redox status. NPs treatment did not induce changes in ATP levels in luteal cells, while it increased (p< 0.05) their proliferation. In endothelial cells, no change in proliferation was detected, while an increase (p<0.05) in ATP levels was observed. The increase of reactive oxygen species, superoxide anion (p<0.05) and nitric oxide (p<0.001) was detected in both cell types, which also showed changes in superoxide dismutase enzyme activity as well as an increase of non-enzymatic antioxidant power (p<0.05). A decrease (p<0.05) in progesterone production as well as an increase of vascular endothelial growth factor A levels were detected (p<0.05). In addition, a dose-dependent accumulation of NPs in endothelial cells was shown, that likely occurred through adhesion and internalization. Results underline potential risk of NPs for corpus luteum functionality.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
