Short-term impact assessment of ocean liming: A copepod exposure test

Ocean liming (OL) is a potential carbon dioxide removal (CDR) method that aims to increase the ocean's capacity to absorb atmospheric CO2 by adding hydrated lime to the surface ocean. Modeling studies indicate that OL may cause temporary pH spikes lasting several minutes, depending on the lime sparging rate. Little is known about the short-term effects of these spikes on marine organisms. Aim of the present study is to investigate these effects on the copepod Acartia tonsa. Copepods were exposed to different pH conditions (9, 10, 11, 12) by dosing different hydrated lime solutions. Copepod mortality, movements, and behavior were recorded. At pH 9 for short exposure times (<6 h), no negative effects were observed indicating a potential tolerable threshold for OL ap-plications. At longer exposure times (>6 h) and pH higher than 9, negative effects (mortality and sublethal ef-fects) were found significantly higher than in the control.