Plastic is an important environmental issue and a more critical aspect concerns plastic fragments, mainly in term of nanoplastics (NPs). We demonstrated that NPs interfere with reproductive and adipose stromal cells. Since several research underlined an increased cardiovascular risk due to NPs, present study was undertaken to investigate their effect on aortic endothelial cells (AOC). We explored the specificity of their interaction with endothelial cells, quantifying their load in treated cells. Then, NPs effect was assessed on cell growth, generation of free radicals and antioxidant defence. Our data demonstrate that NPs colocalize with AOC. We found a significant (p<0.01) increase both in metabolic activity and Vascular Endothelial Growth Factor (VEGF) production (p<0.01). Redox status appeared to be disrupted (p<0.05) by NPS. Taken together, the normal function of cultured AOC appeared negatively affected by AOC. Since NPs have been detected in blood, our present data appear of particular interest.
Are the new phthalates safe? Evaluation of Diisononilphtalate (DINP) effects in porcine ovarian cell cultures / Basini, G; Bussolati, S; Grolli, S; Berni, P; Grasselli, F. - In: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY. - ISSN 1382-6689. - 106:(2024), pp. 1-6. [10.1016/j.etap.2024.104384]
Are the new phthalates safe? Evaluation of Diisononilphtalate (DINP) effects in porcine ovarian cell cultures
G Basini
;S Bussolati;S Grolli;P Berni;F Grasselli
2024-01-01
Abstract
Plastic is an important environmental issue and a more critical aspect concerns plastic fragments, mainly in term of nanoplastics (NPs). We demonstrated that NPs interfere with reproductive and adipose stromal cells. Since several research underlined an increased cardiovascular risk due to NPs, present study was undertaken to investigate their effect on aortic endothelial cells (AOC). We explored the specificity of their interaction with endothelial cells, quantifying their load in treated cells. Then, NPs effect was assessed on cell growth, generation of free radicals and antioxidant defence. Our data demonstrate that NPs colocalize with AOC. We found a significant (p<0.01) increase both in metabolic activity and Vascular Endothelial Growth Factor (VEGF) production (p<0.01). Redox status appeared to be disrupted (p<0.05) by NPS. Taken together, the normal function of cultured AOC appeared negatively affected by AOC. Since NPs have been detected in blood, our present data appear of particular interest.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
