We investigated the effect of chronic cigarette smoking on the expression of haem-oxygenase (HO)-1 and HO-2. Normal subjects and asymptomatic young current smokers with normal lung function tests underwent bronchoalveolar lavage for recovery of macrophages. Reverse transcription/polymerase chain reaction (RT-PCR) analysis showed no significant difference in HO-1 and HO-2 mRNA expression between the two groups. On the other hand, Western blot analysis showed a significant (P<0.05) reduction of HO-2 protein, but not of HO-1, in alveolar macrophages from smokers compared to normal. There was no significant differences by immunocytochemistry for HO-1 and HO-2 expression between the groups. We concluded that HO-2 expression is reduced in alveolar macrophages of smokers, possibly due to the oxidative stress of cigarette smoke. This may in turn lead to reduced protection against further oxidative insults.

Effect of cigarette smoking on haem-oxygenase expression in alveolar macrophages / Atzori, L; Caramori, Gaetano; Lim, S; Jazrawi, E; Donnelly, L; Adcock, I; Barnes, Pj; Chung, Kf. - In: RESPIRATORY MEDICINE. - ISSN 0954-6111. - 98:6(2004), pp. 530-535.

Effect of cigarette smoking on haem-oxygenase expression in alveolar macrophages

CARAMORI, Gaetano;
2004-01-01

Abstract

We investigated the effect of chronic cigarette smoking on the expression of haem-oxygenase (HO)-1 and HO-2. Normal subjects and asymptomatic young current smokers with normal lung function tests underwent bronchoalveolar lavage for recovery of macrophages. Reverse transcription/polymerase chain reaction (RT-PCR) analysis showed no significant difference in HO-1 and HO-2 mRNA expression between the two groups. On the other hand, Western blot analysis showed a significant (P<0.05) reduction of HO-2 protein, but not of HO-1, in alveolar macrophages from smokers compared to normal. There was no significant differences by immunocytochemistry for HO-1 and HO-2 expression between the groups. We concluded that HO-2 expression is reduced in alveolar macrophages of smokers, possibly due to the oxidative stress of cigarette smoke. This may in turn lead to reduced protection against further oxidative insults.
2004
Effect of cigarette smoking on haem-oxygenase expression in alveolar macrophages / Atzori, L; Caramori, Gaetano; Lim, S; Jazrawi, E; Donnelly, L; Adcock, I; Barnes, Pj; Chung, Kf. - In: RESPIRATORY MEDICINE. - ISSN 0954-6111. - 98:6(2004), pp. 530-535.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2964129
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