Sedum telephium ssp. maximum is a medicinal plant used in popular medicine to cure several inflammatory skin diseases, it appears to possess anti-inflammatory, analgesic and keratolytic properties. In the present study, we aimed to investigate the anti-inflammatory activity of the methanol extract of Sedum telephium ssp. maximum (STME) in rat peritoneal macrophages (M@s) stimulated with lipopolysaccharide (LPS) from Salmonella enteritidis. After stimulation with 10 Ag/ml of LPS, peritoneal rat M@s were co-incubated with different doses of STME (8 to 32Ag/ml) or RPMI medium alone using different times of incubation. Treatment of STME reduced mRNA levels of tumor necrosis factor-E and significantly inhibited inducible-nitric oxide synthase (iNOS) expression. Moreover, STME blunted nitrite release and significantly inhibited both extracellular signal regulated kinase 1/2 and c-Jun N-terminal kinase activation in LPS-stimulated M@sG. These data, collectively, show that the methanol extract of Sedum telephium ssp. maximum has an inhibitory activity on mitogen activated protein kinases activation and downstream TNF-E and iNOS production, suggesting that this phytoextract might be useful as a potential antiinflammatory agent.
Anti-inflammatory effects of the methanol extract of Sedum telephium ssp. maximum in lipopolysaccharide- stimulated rat peritoneal macrophages / Altavilla, D.; Polito, F.; Bitto, A.; Minutoli, L.; Miraldi, E.; Fiumara, T.; Biagi, M.; Marini, H.; Giachetti, D.; Vaccaro, M.; Squadrito, F.. - In: PHARMACOLOGY. - ISSN 0031-7012. - 82:4(2008), pp. 250-256. [10.1159/000157626]
Anti-inflammatory effects of the methanol extract of Sedum telephium ssp. maximum in lipopolysaccharide- stimulated rat peritoneal macrophages
BIAGI, M.;
2008-01-01
Abstract
Sedum telephium ssp. maximum is a medicinal plant used in popular medicine to cure several inflammatory skin diseases, it appears to possess anti-inflammatory, analgesic and keratolytic properties. In the present study, we aimed to investigate the anti-inflammatory activity of the methanol extract of Sedum telephium ssp. maximum (STME) in rat peritoneal macrophages (M@s) stimulated with lipopolysaccharide (LPS) from Salmonella enteritidis. After stimulation with 10 Ag/ml of LPS, peritoneal rat M@s were co-incubated with different doses of STME (8 to 32Ag/ml) or RPMI medium alone using different times of incubation. Treatment of STME reduced mRNA levels of tumor necrosis factor-E and significantly inhibited inducible-nitric oxide synthase (iNOS) expression. Moreover, STME blunted nitrite release and significantly inhibited both extracellular signal regulated kinase 1/2 and c-Jun N-terminal kinase activation in LPS-stimulated M@sG. These data, collectively, show that the methanol extract of Sedum telephium ssp. maximum has an inhibitory activity on mitogen activated protein kinases activation and downstream TNF-E and iNOS production, suggesting that this phytoextract might be useful as a potential antiinflammatory agent.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
