Antimicrobial resistance is a major public health concern worldwide. Albeit to a lesser extent than bacteria, fungi are also becoming increasingly resistant to antifungal drugs. Moreover, due to the small number of antifungal classes, therapy options are limited, complicating the clinical management of mycoses. In this view, antimicrobial peptides (AMPs) are a potential alternative to conventional drugs. Among these, Proline-rich antimicrobial peptides (PrAMPs), almost exclusively of animal origins, are of particular interest due to their peculiar mode of action. In this study, a search for new arginine- and proline-rich peptides from plants has been carried out with a bioinformatic approach by sequence alignment and antimicrobial prediction tools. Two peptide candidates were tested against planktonic cells and biofilms of Candida albicans and Candida glabrata strains, including resistant isolates. These peptides showed similar potent activity, with half-maximal effective concentration values in the micromolar range. In addition, some structural and functional features, revealing peculiar mechanistic behaviors, were investigated.

Novel Arginine- and Proline-Rich Candidacidal Peptides Obtained through a Bioinformatic Approach / Ciociola, Tecla; Giovati, Laura; De Simone, Tiziano; Bergamaschi, Greta; Gori, Alessandro; Consalvi, Valerio; Conti, Stefania; Vitali, Alberto. - In: ANTIBIOTICS. - ISSN 2079-6382. - 12:3(2023), p. 472.472. [10.3390/antibiotics12030472]

Novel Arginine- and Proline-Rich Candidacidal Peptides Obtained through a Bioinformatic Approach

Ciociola, Tecla
Conceptualization
;
Giovati, Laura
Conceptualization
;
De Simone, Tiziano
Investigation
;
Conti, Stefania
Writing – Review & Editing
;
2023-01-01

Abstract

Antimicrobial resistance is a major public health concern worldwide. Albeit to a lesser extent than bacteria, fungi are also becoming increasingly resistant to antifungal drugs. Moreover, due to the small number of antifungal classes, therapy options are limited, complicating the clinical management of mycoses. In this view, antimicrobial peptides (AMPs) are a potential alternative to conventional drugs. Among these, Proline-rich antimicrobial peptides (PrAMPs), almost exclusively of animal origins, are of particular interest due to their peculiar mode of action. In this study, a search for new arginine- and proline-rich peptides from plants has been carried out with a bioinformatic approach by sequence alignment and antimicrobial prediction tools. Two peptide candidates were tested against planktonic cells and biofilms of Candida albicans and Candida glabrata strains, including resistant isolates. These peptides showed similar potent activity, with half-maximal effective concentration values in the micromolar range. In addition, some structural and functional features, revealing peculiar mechanistic behaviors, were investigated.
2023
Novel Arginine- and Proline-Rich Candidacidal Peptides Obtained through a Bioinformatic Approach / Ciociola, Tecla; Giovati, Laura; De Simone, Tiziano; Bergamaschi, Greta; Gori, Alessandro; Consalvi, Valerio; Conti, Stefania; Vitali, Alberto. - In: ANTIBIOTICS. - ISSN 2079-6382. - 12:3(2023), p. 472.472. [10.3390/antibiotics12030472]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2941958
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