Introduction Histiocytic sarcoma (HS) is a highly aggressive neoplasm with a limited response to therapies. A promising new approach might be oncolytic virotherapy. Angiogenesis is essential for tumor growth and metastasis. One of the key regulator factors for angiogenesis is hypoxia inducible factor-1α (HIF-1α). In addition, angiogenesis is regulated by reactive oxygen species (ROS). The aim of this study was to evaluate the influence of canine distemper virus (CDV) infection on HIF-1α expression in canine HS cells (DH82 cells). Materials and methods Non-infected and persistently CDV infected DH82 cells were investigated in vitro. The expression of oxidative stress and angiogenesis markers were evaluated on a molecular and protein level using microarray data, immunofluorescence microscopy, Western blot and flow cytometry. Results Persistently CDV-infected DH82 cells displayed an increased oxidative stress due to viral infection that leads to a dysregulated HIF-1α expression with a consecutive downregulation of the downstream angiogenetic pathway. Conclusion These results indicate that a persistent CDV-infection seems to affect the HIF-1α pathway resulting in a decrease in the downstream production of angiogenetic factors in vitro.

Oxidative stress in canine histiocytic sarcoma cells (DH82 cells) induced by a persistent canine distemper virus infection leads to impairment of the HIF-1α downstream pathway in vitro / Armando, F; Gambini, M; Corradi, A; Giudice, C; Pfankuche, Vm; Brogden, G; Attig, F; von Köckritz-Blickwede, M; Baumgärtner, W; Puff, C. - In: TIERARZTLICHE PRAXIS AUSGABE KLEINTIERE HEIMTIERE. - ISSN 1434-1239. - 48:3(2020), pp. 216-216. [10.1055/s-0040-1712568]

Oxidative stress in canine histiocytic sarcoma cells (DH82 cells) induced by a persistent canine distemper virus infection leads to impairment of the HIF-1α downstream pathway in vitro

F Armando
Conceptualization
;
A Corradi
Writing – Review & Editing
;
2020-01-01

Abstract

Introduction Histiocytic sarcoma (HS) is a highly aggressive neoplasm with a limited response to therapies. A promising new approach might be oncolytic virotherapy. Angiogenesis is essential for tumor growth and metastasis. One of the key regulator factors for angiogenesis is hypoxia inducible factor-1α (HIF-1α). In addition, angiogenesis is regulated by reactive oxygen species (ROS). The aim of this study was to evaluate the influence of canine distemper virus (CDV) infection on HIF-1α expression in canine HS cells (DH82 cells). Materials and methods Non-infected and persistently CDV infected DH82 cells were investigated in vitro. The expression of oxidative stress and angiogenesis markers were evaluated on a molecular and protein level using microarray data, immunofluorescence microscopy, Western blot and flow cytometry. Results Persistently CDV-infected DH82 cells displayed an increased oxidative stress due to viral infection that leads to a dysregulated HIF-1α expression with a consecutive downregulation of the downstream angiogenetic pathway. Conclusion These results indicate that a persistent CDV-infection seems to affect the HIF-1α pathway resulting in a decrease in the downstream production of angiogenetic factors in vitro.
2020
Oxidative stress in canine histiocytic sarcoma cells (DH82 cells) induced by a persistent canine distemper virus infection leads to impairment of the HIF-1α downstream pathway in vitro / Armando, F; Gambini, M; Corradi, A; Giudice, C; Pfankuche, Vm; Brogden, G; Attig, F; von Köckritz-Blickwede, M; Baumgärtner, W; Puff, C. - In: TIERARZTLICHE PRAXIS AUSGABE KLEINTIERE HEIMTIERE. - ISSN 1434-1239. - 48:3(2020), pp. 216-216. [10.1055/s-0040-1712568]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2939291
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact