A novel liquid chromatography-mass spectrometry (LC-MS) interfacing concept is presented and discussed. The new interface, called Liquid-EI (LEI), is based on electron ionization (EI) but, differently from any previous attempt, the vaporization of solutes and mobile phase takes place at atmospheric pressure into a specifically designed region, called “vaporization micro-channel”, before entering the high-vacuum ion source. The interface is completely independent from the rest of the instrumentation, and can be adapted to any gas chromatography-mass spectrometry (GC-MS) system, as an add-on for a rapid LC-MS conversion. Pressure drop and temperature gradient between LC and MS were considered to enhance the analyte response and reduce band broadening and/or solute carryovers. A fused silica liner, placed inside the vaporization micro-channel, acts as an inert vaporization surface speeding up the gas-phase conversion of large molecules while lessening possible memory effects. The liner is easily replaceable for a quick and extremely simple interface maintenance. Proof of concept and detailed description of the interface are here presented.
Atmospheric Pressure Vaporization Mechanism for Coupling a Liquid Phase with Electron Ionization Mass Spectrometry / Termopoli, Veronica; Famiglini, Giorgio; Palma, Pierangela; Piergiovanni, Maurizio; Cappiello, Achille. - In: ANALYTICAL CHEMISTRY. - ISSN 1520-6882. - 89:3(2017), pp. 2049-2056. [10.1021/acs.analchem.6b04646]
Atmospheric Pressure Vaporization Mechanism for Coupling a Liquid Phase with Electron Ionization Mass Spectrometry
PIERGIOVANNI, MAURIZIO;CAPPIELLO, ACHILLE
2017-01-01
Abstract
A novel liquid chromatography-mass spectrometry (LC-MS) interfacing concept is presented and discussed. The new interface, called Liquid-EI (LEI), is based on electron ionization (EI) but, differently from any previous attempt, the vaporization of solutes and mobile phase takes place at atmospheric pressure into a specifically designed region, called “vaporization micro-channel”, before entering the high-vacuum ion source. The interface is completely independent from the rest of the instrumentation, and can be adapted to any gas chromatography-mass spectrometry (GC-MS) system, as an add-on for a rapid LC-MS conversion. Pressure drop and temperature gradient between LC and MS were considered to enhance the analyte response and reduce band broadening and/or solute carryovers. A fused silica liner, placed inside the vaporization micro-channel, acts as an inert vaporization surface speeding up the gas-phase conversion of large molecules while lessening possible memory effects. The liner is easily replaceable for a quick and extremely simple interface maintenance. Proof of concept and detailed description of the interface are here presented.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.