Single cell classification is elucidating homeostasis and pathology in tissues and whole organs. We applied in situ spatial proteomics by multiplex antibody staining to routinely processed mouse lung, healthy and during a fibrosis model. With a limited validated antibody panel (24) we classify the normal constituents (alveolar type I and II, bronchial epithelia, endothelial, muscular, stromal and hematopoietic cells) and by quantitative measurements, we show the progress of lung fibrosis over a 4 weeks course, the changing landscape and the cell-specific quantitative variation of a multidrug transporter. An early decline in AT2 alveolar cells and a progressive increase in stromal cells seems at the core of the fibrotic process.

The normal and fibrotic mouse lung classified by spatial proteomic analysis / Ciccimarra, Roberta; Bolognesi, Maddalena M; Zoboli, Matteo; Cattoretti, Giorgio; Stellari, Franco F; Ravanetti, Francesca. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 12:1(2022), p. 8742. [10.1038/s41598-022-12738-9]

The normal and fibrotic mouse lung classified by spatial proteomic analysis

Ciccimarra, Roberta;Zoboli, Matteo;Ravanetti, Francesca
2022

Abstract

Single cell classification is elucidating homeostasis and pathology in tissues and whole organs. We applied in situ spatial proteomics by multiplex antibody staining to routinely processed mouse lung, healthy and during a fibrosis model. With a limited validated antibody panel (24) we classify the normal constituents (alveolar type I and II, bronchial epithelia, endothelial, muscular, stromal and hematopoietic cells) and by quantitative measurements, we show the progress of lung fibrosis over a 4 weeks course, the changing landscape and the cell-specific quantitative variation of a multidrug transporter. An early decline in AT2 alveolar cells and a progressive increase in stromal cells seems at the core of the fibrotic process.
The normal and fibrotic mouse lung classified by spatial proteomic analysis / Ciccimarra, Roberta; Bolognesi, Maddalena M; Zoboli, Matteo; Cattoretti, Giorgio; Stellari, Franco F; Ravanetti, Francesca. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 12:1(2022), p. 8742. [10.1038/s41598-022-12738-9]
File in questo prodotto:
File Dimensione Formato  
41598_2022_Article_12738.pdf

accesso aperto

Tipologia: Versione (PDF) editoriale
Licenza: Creative commons
Dimensione 6.94 MB
Formato Adobe PDF
6.94 MB Adobe PDF Visualizza/Apri
41598_2022_12738_MOESM1_ESM.docx

accesso aperto

Tipologia: Altro materiale allegato
Licenza: Creative commons
Dimensione 8.71 MB
Formato Microsoft Word XML
8.71 MB Microsoft Word XML Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2926691
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact