Influenza recombinant proteins and virus-like particles (VLPs) play an important role in vaccine development (e.g., CadiFlu-S). However, their production from mammalian cells suffers from low yields and lack of control of the final VLPs. To improve these issues, characterization techniques able to visualize and quantify the different steps of the process are needed. Fluorescence microscopy represents a powerful tool able to image multiple protein targets; however, its limited resolution hinders the study of viral constructs. Here, we propose the use of super-resolution microscopy and in particular of DNA-point accumulation for imaging in nanoscale topography (DNA-PAINT) microscopy as a characterization method for recombinant viral proteins on both cells and VLPs. We were able to quantify the amount of the three main influenza proteins (hemagglutinin (HA), neuraminidase (NA), and ion channel matrix protein 2 (M2)) per cell and per VLP with nanometer resolution and single-molecule sensitivity, proving that DNA-PAINT is a powerful technique to characterize recombinant viral constructs.

Nanoscale Mapping of Recombinant Viral Proteins: From Cells to Virus-Like Particles / Arista-Romero, M.; Delcanale, P.; Pujals, S.; Albertazzi, L.. - In: ACS PHOTONICS. - ISSN 2330-4022. - (2021). [10.1021/acsphotonics.1c01154]

Nanoscale Mapping of Recombinant Viral Proteins: From Cells to Virus-Like Particles

Delcanale P.;
2021

Abstract

Influenza recombinant proteins and virus-like particles (VLPs) play an important role in vaccine development (e.g., CadiFlu-S). However, their production from mammalian cells suffers from low yields and lack of control of the final VLPs. To improve these issues, characterization techniques able to visualize and quantify the different steps of the process are needed. Fluorescence microscopy represents a powerful tool able to image multiple protein targets; however, its limited resolution hinders the study of viral constructs. Here, we propose the use of super-resolution microscopy and in particular of DNA-point accumulation for imaging in nanoscale topography (DNA-PAINT) microscopy as a characterization method for recombinant viral proteins on both cells and VLPs. We were able to quantify the amount of the three main influenza proteins (hemagglutinin (HA), neuraminidase (NA), and ion channel matrix protein 2 (M2)) per cell and per VLP with nanometer resolution and single-molecule sensitivity, proving that DNA-PAINT is a powerful technique to characterize recombinant viral constructs.
Nanoscale Mapping of Recombinant Viral Proteins: From Cells to Virus-Like Particles / Arista-Romero, M.; Delcanale, P.; Pujals, S.; Albertazzi, L.. - In: ACS PHOTONICS. - ISSN 2330-4022. - (2021). [10.1021/acsphotonics.1c01154]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2911941
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