The use of alloplastic materials in periodontal regenerative therapies is limited by their incapacity to establish a dynamic dialog with the surrounding milieu. The aim of the present study was to control biomaterial surface bioactivity by introducing aptamers to induce the selective adsorption of fibronectin from blood, thus promoting platelets activation in vitro and bone regeneration in vivo. A hyaluronic acid/polyethyleneglycole-based hydrogel was enriched with aptamers selected for recognizing and binding fibronectin. In vitro, the capacity of constructs to support osteoblast adhesion, as well as platelets aggregation and activation was assessed by chemiluminescence within 24h. Matrices were then evaluated in a rat periodontal defect to assess their regenerative potential by microcomputed tomography (CT) and their osteogenic capacity by Luminex assay 5, 15 and 30 days postoperatively. Aptamers were found to confer matrices the capacity of sustaining firm cell adhesion and to promote platelets activation. In vivo, aptamers promoted new bone formation by enhancing osteoblastic lineage commitment maturation. Aptamers are a viable surface modification, which confers alloplastic materials the potential capacity to orchestrate blood clot formation, thus controlling bone healing.

Aptamers recognizing fibronectin confer improved bioactivity to biomaterials and promote new bone formation in a periodontal defect in rat / Parisi, Ludovica; Rivara, Federico; Alves Costa, Camila; Paolo Flores Abuna, Rodriguo; Bazan Palioto, Daniela; Macaluso, Guido Maria. - In: BIOMEDICAL MATERIALS. - ISSN 1748-6041. - (2020). [10.1088/1748-605X/abb6b2]

Aptamers recognizing fibronectin confer improved bioactivity to biomaterials and promote new bone formation in a periodontal defect in rat

Ludovica Parisi
Membro del Collaboration Group
;
Federico Rivara
Membro del Collaboration Group
;
Guido Maria Macaluso
2020

Abstract

The use of alloplastic materials in periodontal regenerative therapies is limited by their incapacity to establish a dynamic dialog with the surrounding milieu. The aim of the present study was to control biomaterial surface bioactivity by introducing aptamers to induce the selective adsorption of fibronectin from blood, thus promoting platelets activation in vitro and bone regeneration in vivo. A hyaluronic acid/polyethyleneglycole-based hydrogel was enriched with aptamers selected for recognizing and binding fibronectin. In vitro, the capacity of constructs to support osteoblast adhesion, as well as platelets aggregation and activation was assessed by chemiluminescence within 24h. Matrices were then evaluated in a rat periodontal defect to assess their regenerative potential by microcomputed tomography (CT) and their osteogenic capacity by Luminex assay 5, 15 and 30 days postoperatively. Aptamers were found to confer matrices the capacity of sustaining firm cell adhesion and to promote platelets activation. In vivo, aptamers promoted new bone formation by enhancing osteoblastic lineage commitment maturation. Aptamers are a viable surface modification, which confers alloplastic materials the potential capacity to orchestrate blood clot formation, thus controlling bone healing.
Aptamers recognizing fibronectin confer improved bioactivity to biomaterials and promote new bone formation in a periodontal defect in rat / Parisi, Ludovica; Rivara, Federico; Alves Costa, Camila; Paolo Flores Abuna, Rodriguo; Bazan Palioto, Daniela; Macaluso, Guido Maria. - In: BIOMEDICAL MATERIALS. - ISSN 1748-6041. - (2020). [10.1088/1748-605X/abb6b2]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2911768
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