Silymarin, a naturally acknowledged hepatoprotector used in humans to treat liver diseases has been tested in murine (HC11) and bovine (BME-UV) mammary epithelial cell lines to evaluate a possible direct effect on cell growth and differentiation in mammary gland. Silymarin enhanced cell proliferation (p < 0.05) from 10 to 1000 ng/ml in association with growth factors, (up to 20%) or alone (up to 15%) versus controls. Furthermore, silymarin (100 ng/ml) was able to increase (p < 0.05) beta-casein gene expression alone or in association with prolactin (5 microg/ml). These effects may be related with protein kinase B (AKT) activation induced by silymarin treatment (p < 0.05) and/or by a dose-related inhibitory effect (p < 0.05) on caspase-3 activity related to a protective role in cell apoptosis. These data suggest that silymarin should be considered a candidate to support mammary gland activity during a lactogenetic state.

Positive effect of silymarin on cell growth and differentiation in bovine and murine mammary cells / Starvaggi Cucuzza, Laura; Motta, M; Miretti, Silvia; Macchi, Elisabetta; Martignani, Eugenio; Accornero, Paolo; Baratta, Mario. - In: JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION. - ISSN 0931-2439. - 94(2010), pp. 111-117. [10.1111/j.1439-0396.2008.00890]

Positive effect of silymarin on cell growth and differentiation in bovine and murine mammary cells

BARATTA, Mario
2010

Abstract

Silymarin, a naturally acknowledged hepatoprotector used in humans to treat liver diseases has been tested in murine (HC11) and bovine (BME-UV) mammary epithelial cell lines to evaluate a possible direct effect on cell growth and differentiation in mammary gland. Silymarin enhanced cell proliferation (p < 0.05) from 10 to 1000 ng/ml in association with growth factors, (up to 20%) or alone (up to 15%) versus controls. Furthermore, silymarin (100 ng/ml) was able to increase (p < 0.05) beta-casein gene expression alone or in association with prolactin (5 microg/ml). These effects may be related with protein kinase B (AKT) activation induced by silymarin treatment (p < 0.05) and/or by a dose-related inhibitory effect (p < 0.05) on caspase-3 activity related to a protective role in cell apoptosis. These data suggest that silymarin should be considered a candidate to support mammary gland activity during a lactogenetic state.
Positive effect of silymarin on cell growth and differentiation in bovine and murine mammary cells / Starvaggi Cucuzza, Laura; Motta, M; Miretti, Silvia; Macchi, Elisabetta; Martignani, Eugenio; Accornero, Paolo; Baratta, Mario. - In: JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION. - ISSN 0931-2439. - 94(2010), pp. 111-117. [10.1111/j.1439-0396.2008.00890]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2899540
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