We previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential to generate a functional mammary outgrowth. The aim of this study was to investigate on the immunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry analysis showed different subpopulations of mammary epithelial cells emerging according to the timing of cell culture. CD49f+-cells significantly increased during the culture (p b 0.01) and a similar trend was observed, even if less regular, for CD29+ and ALDH1 positive cell populations. No difference during the culture was observed for CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative capabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when transplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation that retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion of the primitive population of adult mammary stem cells.

Bovine CD49 positive-cell subpopulation remarkably increases in mammary epithelial cells that retain a stem-like phenotype / Cravero, Diego; Martignani, Eugenio; Miretti, Silvia; Accornero, Paolo; Baratta, Mario. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 102(2015), pp. 1-6. [10.1016/j.rvsc.2015.07.003]

Bovine CD49 positive-cell subpopulation remarkably increases in mammary epithelial cells that retain a stem-like phenotype

BARATTA, Mario
2015

Abstract

We previously proved that adult stem cells reside in the bovine mammary gland and possess an intrinsic potential to generate a functional mammary outgrowth. The aim of this study was to investigate on the immunophenotyping features retained by mammary stem-like cells detected in long term culture. Flow cytometry analysis showed different subpopulations of mammary epithelial cells emerging according to the timing of cell culture. CD49f+-cells significantly increased during the culture (p b 0.01) and a similar trend was observed, even if less regular, for CD29+ and ALDH1 positive cell populations. No difference during the culture was observed for CD24 positive cells but after 35 days of culture a subset of cells, CD49f positive, still retained regenerative capabilities in in vivo xenotransplants. These cells were able to form organized pseudo-alveoli when transplanted in immunodeficient mice. These results prove the presence of a multipotent cell subpopulation that retain a strong epithelial induction, confirmed in in vivo xenotransplants with a presumable in vitro expansion of the primitive population of adult mammary stem cells.
Bovine CD49 positive-cell subpopulation remarkably increases in mammary epithelial cells that retain a stem-like phenotype / Cravero, Diego; Martignani, Eugenio; Miretti, Silvia; Accornero, Paolo; Baratta, Mario. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 102(2015), pp. 1-6. [10.1016/j.rvsc.2015.07.003]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2899525
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