The in vitro and in vivo assays have been developed to detect primitive bovine mammary cells. The in vitro CFC assay allowed us to detect different type of progenitors as demonstrated by the different colonies that they were able to generate when cultured in vitro. Cells from bovine mammary tissue were able to generate organized outgrowths in a xenograft model. Moreover progenitor cells could be detected in these outgrowths, but the frequencies of the different type of colonies showed a marked variation when compared to the CFC assay performed on freshly dissociated BMECs. The systems described here provide very useful tools to improve the knowledge of the bovine mammary tissue hierarchy and of the mechanisms and factors that drive proliferation and differentiation. As future perspective this knowledge may then be used to develop new strategies for increasing milk production in dairy cows by specifically targeting signaling pathways that are important in the control of the stem/progenitor cell compartment

Functional identification of bovine mammary epithelial stem/progenitor cells / Baratta, Mario; Martignani, Eugenio; Eaves, C.. - (2007). [2433413]

Functional identification of bovine mammary epithelial stem/progenitor cells

BARATTA, Mario;
2007

Abstract

The in vitro and in vivo assays have been developed to detect primitive bovine mammary cells. The in vitro CFC assay allowed us to detect different type of progenitors as demonstrated by the different colonies that they were able to generate when cultured in vitro. Cells from bovine mammary tissue were able to generate organized outgrowths in a xenograft model. Moreover progenitor cells could be detected in these outgrowths, but the frequencies of the different type of colonies showed a marked variation when compared to the CFC assay performed on freshly dissociated BMECs. The systems described here provide very useful tools to improve the knowledge of the bovine mammary tissue hierarchy and of the mechanisms and factors that drive proliferation and differentiation. As future perspective this knowledge may then be used to develop new strategies for increasing milk production in dairy cows by specifically targeting signaling pathways that are important in the control of the stem/progenitor cell compartment
Functional identification of bovine mammary epithelial stem/progenitor cells / Baratta, Mario; Martignani, Eugenio; Eaves, C.. - (2007). [2433413]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2899453
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