We present an in vitro study of lung epithelial and fibroblastic cell lines exposed to growing concentrations (5%, 10%) of cigarette smoke extract (CSE), and to N-acetylcysteine. The heme oxygenase 1 (HO-1) gene expression was stimulated by CSE in a dose-dependent manner in both immortalized bronchial epithelium (BEAS 2B) and foetal pulmonary fibroblasts (HFL1), whereas no effect was seen in lung adenocarcinoma cells (A549). HO-1 gene expression, differently regulated in the three cell lines, seems to be provoked by CSE induced oxidative stress.

In vitro characterization of heme oxygenase 1 in human epithelial lung cells exposed to cigarette smoke [Caratterizzazione in vitro dell'espressione dell'eme ossigenasi 1 in cellule epiteliali polmonari umane esposte a fumo di sigaretta] / Scotti, E.; De Palma, G.; Mozzoni, P.; Carnevali, S.; Luppi, F.; Caglieri, A.; Vettori, M. V.; Goldoni, M.; Fabbri, L.; Mutti, A.. - In: GIORNALE ITALIANO DI MEDICINA DEL LAVORO ED ERGONOMIA. - ISSN 1592-7830. - 26:4(2004), pp. 103-104.

In vitro characterization of heme oxygenase 1 in human epithelial lung cells exposed to cigarette smoke [Caratterizzazione in vitro dell'espressione dell'eme ossigenasi 1 in cellule epiteliali polmonari umane esposte a fumo di sigaretta]

Mozzoni P.;Goldoni M.;
2004

Abstract

We present an in vitro study of lung epithelial and fibroblastic cell lines exposed to growing concentrations (5%, 10%) of cigarette smoke extract (CSE), and to N-acetylcysteine. The heme oxygenase 1 (HO-1) gene expression was stimulated by CSE in a dose-dependent manner in both immortalized bronchial epithelium (BEAS 2B) and foetal pulmonary fibroblasts (HFL1), whereas no effect was seen in lung adenocarcinoma cells (A549). HO-1 gene expression, differently regulated in the three cell lines, seems to be provoked by CSE induced oxidative stress.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2896138
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