Numerous metagenome-wide association studies (MWAS) for urolithiasis have been published, leading to the discovery of potential interactions between the microbiome and urolithiasis. However, questions remain about the reproducibility, applicability and physiological relevance of these data owing to discrepancies in experimental technique and a lack of standardization in the field. One barrier to interpreting MWAS is that experimental biases can be introduced at every step of the experimental pipeline, including sample collection, preservation, storage, processing, sequencing, data analysis and validation. Thus, the introduction of standardized protocols that maintain the flexibility to achieve study-specific objectives is urgently required. To address this need, the first international consortium for microbiome in urinary stone disease - MICROCOSM - was created and consensus panel members were asked to participate in a consensus meeting to develop standardized protocols for microbiome studies if they had published an MWAS on urolithiasis. Study-specific protocols were revised until a consensus was reached. This consensus group generated standardized protocols, which are publicly available via a secure online server, for each step in the typical clinical microbiome-urolithiasis study pipeline. This standardization creates the benchmark for future studies to facilitate consistent interpretation of results and, collectively, to lead to effective interventions to prevent the onset of urolithiasis, and will also be useful for investigators interested in microbiome research in other urological diseases.

Standardization of microbiome studies for urolithiasis: an international consensus agreement / Kachroo, Naveen; Lange, Dirk; Penniston, Kristina L; Stern, Joshua; Tasian, Gregory; Bajic, Petar; Wolfe, Alan J; Suryavanshi, Mangesh; Ticinesi, Andrea; Meschi, Tiziana; Monga, Manoj; Miller, Aaron W. - In: NATURE REVIEWS. UROLOGY. - ISSN 1759-4812. - 18:5(2021), pp. 303-311. [10.1038/s41585-021-00450-8]

Standardization of microbiome studies for urolithiasis: an international consensus agreement

Ticinesi, Andrea;Meschi, Tiziana;
2021

Abstract

Numerous metagenome-wide association studies (MWAS) for urolithiasis have been published, leading to the discovery of potential interactions between the microbiome and urolithiasis. However, questions remain about the reproducibility, applicability and physiological relevance of these data owing to discrepancies in experimental technique and a lack of standardization in the field. One barrier to interpreting MWAS is that experimental biases can be introduced at every step of the experimental pipeline, including sample collection, preservation, storage, processing, sequencing, data analysis and validation. Thus, the introduction of standardized protocols that maintain the flexibility to achieve study-specific objectives is urgently required. To address this need, the first international consortium for microbiome in urinary stone disease - MICROCOSM - was created and consensus panel members were asked to participate in a consensus meeting to develop standardized protocols for microbiome studies if they had published an MWAS on urolithiasis. Study-specific protocols were revised until a consensus was reached. This consensus group generated standardized protocols, which are publicly available via a secure online server, for each step in the typical clinical microbiome-urolithiasis study pipeline. This standardization creates the benchmark for future studies to facilitate consistent interpretation of results and, collectively, to lead to effective interventions to prevent the onset of urolithiasis, and will also be useful for investigators interested in microbiome research in other urological diseases.
Standardization of microbiome studies for urolithiasis: an international consensus agreement / Kachroo, Naveen; Lange, Dirk; Penniston, Kristina L; Stern, Joshua; Tasian, Gregory; Bajic, Petar; Wolfe, Alan J; Suryavanshi, Mangesh; Ticinesi, Andrea; Meschi, Tiziana; Monga, Manoj; Miller, Aaron W. - In: NATURE REVIEWS. UROLOGY. - ISSN 1759-4812. - 18:5(2021), pp. 303-311. [10.1038/s41585-021-00450-8]
File in questo prodotto:
File Dimensione Formato  
Kachroo Nat Rev Urol.pdf

accesso aperto

Descrizione: Pdf pubblicato in Open Access
Tipologia: Versione (PDF) editoriale
Licenza: Creative commons
Dimensione 1.41 MB
Formato Adobe PDF
1.41 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2891439
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 4
social impact