Naturally contaminated and artificially Fusarium spp. inoculated wheat was analyzed for deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol. After extraction and clean-up with MycoSep columns, the trichothecenes were determined using a LC-ESI-MS/MS method. Deoxynivalenol-3-glucoside was detectable in 4 out of 4 artificially inoculated and in 22 out of 25 naturally contaminated wheat samples. For the latter, the average relative deoxynivalenol-3-glucoside concentration was about 6 % of the deoxynivalenol concentration. The maximum relative deoxynivalenol-3-glucoside concentration was 12% as compared to the concentration of deoxynivalenol. In all samples, the concentration of deoxynivalenol-3-glucoside was higher than the concentrations of 3-acetyl-deoxynivalenol or 15-acetyl-deoxynivalenol.

Determination of DON-3-glucoside in artificially and naturally contaminated wheat with LC-MS/MS / Berthiller, F.; Krska, R.; Dall'Asta, C.; Lemmens, M.; Adam, G.; Schuhmacher, R.. - In: MYCOTOXIN RESEARCH. - ISSN 0178-7888. - 21:3(2005), pp. 205-208. [10.1007/BF02959264]

Determination of DON-3-glucoside in artificially and naturally contaminated wheat with LC-MS/MS

Dall'Asta C.;
2005-01-01

Abstract

Naturally contaminated and artificially Fusarium spp. inoculated wheat was analyzed for deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol. After extraction and clean-up with MycoSep columns, the trichothecenes were determined using a LC-ESI-MS/MS method. Deoxynivalenol-3-glucoside was detectable in 4 out of 4 artificially inoculated and in 22 out of 25 naturally contaminated wheat samples. For the latter, the average relative deoxynivalenol-3-glucoside concentration was about 6 % of the deoxynivalenol concentration. The maximum relative deoxynivalenol-3-glucoside concentration was 12% as compared to the concentration of deoxynivalenol. In all samples, the concentration of deoxynivalenol-3-glucoside was higher than the concentrations of 3-acetyl-deoxynivalenol or 15-acetyl-deoxynivalenol.
2005
Determination of DON-3-glucoside in artificially and naturally contaminated wheat with LC-MS/MS / Berthiller, F.; Krska, R.; Dall'Asta, C.; Lemmens, M.; Adam, G.; Schuhmacher, R.. - In: MYCOTOXIN RESEARCH. - ISSN 0178-7888. - 21:3(2005), pp. 205-208. [10.1007/BF02959264]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2885226
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 11
  • ???jsp.display-item.citation.isi??? ND
social impact