Based on our previous study in follicle, the first aim of the work was to evaluate the effect of melatonin in the swine corpus luteum. Luteal cells were exposed to 10 and 20 pg mL-1 melatonin. We evaluated the effect on proliferation (Bromo-deoxy-Uridine uptake), steroidogenesis (progesterone) and redox status by means of Griess test (nitric oxide production), WST-1 test (superoxide anion generation) and FRAP test (non-enzymatic antioxidant power). The results show a significant increase of the antioxidant power as well as a reduction of the other parameters analyzed. These data and the expression of MT2 observed in luteal cells, allow us to hypothesize a physiological role of melatonin in the regulation of corpus luteum functionality. The reproductive function is dependent on energy reserves stored in adipose tissue. Therefore, we sought to verify effect of melatonin on adipose stromal cells (ASCs). MT2 receptor expression was detected in ASCs and the presence of gene markers (PPARγ and leptin) before and after adipogenic differentiation was verified. The differentiation was significantly inhibited by melatonin, as well as cell viability. In conclusion, present results suggest that melatonin exerts a potential inhibitory action on luteal function and adipogenesis, possibly mediated by MT2.
Melatonin modulates swine luteal and adipose stromal cell functions / Dodi, Alessandra; Bussolati, Simona; Grolli, Stefano; Grasselli, Francesca; Di Lecce, Rosanna; Basini, Giuseppina. - In: REPRODUCTION FERTILITY AND DEVELOPMENT. - ISSN 1031-3613. - 33:3(2021), pp. 198-208. [doi: 10.1071/RD20312]
|Appare nelle tipologie:||1.1 Articolo su rivista|