Epigenetic reprogramming by adenovirus e1a

Adenovirus e1a induces quiescent human cells to replicate We found that e1a causes global relocalization of the RB ( retinoblastoma) proteins ( RB p130 and p107) and p300/ CBP histone acetyltransferases on promoters the effect of which is to restrict the acetylation of histone 3 lysine- 18 ( H3K18ac) to a limited set of genes thereby stimulating cell cycling and inhibiting antiviral responses and cellular differentiation Soon after expression e1a binds transiently to promoters of cell cycle and growth genes causing enrichment of p300/ CBP PCAF ( p300/ CBP- associated factor) and H3K18ac; depletion of RB proteins; and transcriptional activation e1a also associates transiently with promoters of antiviral genes causing enrichment for RB p130 and H4K16ac; increased nucleosome density; and transcriptional repression At later times e1a and p107 bind mainly to promoters of development and differentiation genes repressing transcription The temporal order of e1a binding requires its interactions with p300/ CBP and RB proteins Our data uncover a defined epigenetic reprogramming leading to cellular transformation