Vascular cells, including smooth muscle cells (VSMC), may release interleukin 1 (IL-1) and transcribe its genes for both isoforms. Previous studies have shown that cysteinyl-leukotrienes can modulate cytokine production by monocytes and a cytokine-eicosanoid network has been suggested during atherosclerosis. In this study the effects of cysteinyl-leukotriene D4 (LTD4) on IL-1β production and IL-1β mRNA expression were tested on rat VSMC. LTD4 showed a significant dose-dependent (from basal production of 55 ± 15 pg/ml to maximal production of 177 ± 14 pg/ml) and time-dependent (peaking at 24 h 16 ± 54 pg/ml) increase of IL-1β immmunoreactivity in the supernatants of conditioned medium and cell lysates. Furthermore, LTD4 induced an increased mRNA expression which began at 1 h and peaked at 12 h incubation time. The production of IL-1β was inhibited by MK-571 (from 145 ± 12 to 60 ± 10 pg/ml), a specific receptor antagonist of LTD4 and partially reduced by IL-1 receptor antagonist (IL-1ra) (from 160 ± 12 to 85 ± 5 pg/ml). These experiments suggest that cysteinyl-leukotrienes, potentially produced in the vascular wall by leukocytes or by trancellular metabolism, may be involved in local IL-1 production. © 1995.
Cysteinyl-leukotriene D4 induced IL-1β expression and release in rat vascular smooth muscle cells / Porreca, ; and Conti, E. a.; B., P.; Feliciani, C.; and Di Febbo, C. c.; and Reale, C. a.; and Mincione, M. b.; and Neri, G. d.; and Amerio, M. a.; and Cuccurullo, P. c.; A., F.. - In: ATHEROSCLEROSIS. - ISSN 0021-9150. - 115:2(1995), pp. 181-189. [10.1016/0021-9150(94)05510-P]
Cysteinyl-leukotriene D4 induced IL-1β expression and release in rat vascular smooth muscle cells
c. FelicianiData Curation
;
1995-01-01
Abstract
Vascular cells, including smooth muscle cells (VSMC), may release interleukin 1 (IL-1) and transcribe its genes for both isoforms. Previous studies have shown that cysteinyl-leukotrienes can modulate cytokine production by monocytes and a cytokine-eicosanoid network has been suggested during atherosclerosis. In this study the effects of cysteinyl-leukotriene D4 (LTD4) on IL-1β production and IL-1β mRNA expression were tested on rat VSMC. LTD4 showed a significant dose-dependent (from basal production of 55 ± 15 pg/ml to maximal production of 177 ± 14 pg/ml) and time-dependent (peaking at 24 h 16 ± 54 pg/ml) increase of IL-1β immmunoreactivity in the supernatants of conditioned medium and cell lysates. Furthermore, LTD4 induced an increased mRNA expression which began at 1 h and peaked at 12 h incubation time. The production of IL-1β was inhibited by MK-571 (from 145 ± 12 to 60 ± 10 pg/ml), a specific receptor antagonist of LTD4 and partially reduced by IL-1 receptor antagonist (IL-1ra) (from 160 ± 12 to 85 ± 5 pg/ml). These experiments suggest that cysteinyl-leukotrienes, potentially produced in the vascular wall by leukocytes or by trancellular metabolism, may be involved in local IL-1 production. © 1995.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
