Background: Direct immunofluorescence (DIF) is a necessary examination tool for the diagnosis of pemphigus. The suction-blister-method splits the skin at the lamina lucida and it is possible with a scalpel to separate the entire epidermis from the dermis. Objective: The study was to determine whether DIF is reliable on epidermal sheets separated using a suction apparatus. Methods: Thirteen patients were selected for this study: (nine with pemphigus vulgaris (PV), one with paraneoplastic pemphigus (PP), and three with pemphigus erythematosus (PE). Frozen epidermal sheets, separated from the dermis with a scalpel, were used as a substrate. Diagnosis with routine fluorescein isothiocyanate (FITC) antibodies was made. Results: In all patients a pericellular deposition of IgG was evident and in eight of these patients a pericellular deposition of C3 was present. In two cases of PE and one of PP, the C3 deposits were also present in the lower part of basal keratinocytes. Conclusion: This diagnostic method without skin biopsy is easy to perform and, together with the histology and clinical aspects, could be a useful tool in the diagnosis of pemphigus. We recommend this method when the patient is allergic to local anaesthetics, the patient easily produces hypertrophic scars, or in follow-up of already biopsied patients.

Direct immunofluorescence diagnosis of pemphigus without biopsy / Feliciani, Claudio; b and Mohammad Pour, C. a.; and Toto, S. a.; and Coscione, P. a.; and Amerio, G. a.; and Amerio, P. a.; P, Asha. - In: JOURNAL OF CUTANEOUS MEDICINE AND SURGERY. - ISSN 1203-4754. - 2:4(1998), pp. 209-211.

Direct immunofluorescence diagnosis of pemphigus without biopsy

Feliciani
Writing – Review & Editing
;
P, ASHA
1998-01-01

Abstract

Background: Direct immunofluorescence (DIF) is a necessary examination tool for the diagnosis of pemphigus. The suction-blister-method splits the skin at the lamina lucida and it is possible with a scalpel to separate the entire epidermis from the dermis. Objective: The study was to determine whether DIF is reliable on epidermal sheets separated using a suction apparatus. Methods: Thirteen patients were selected for this study: (nine with pemphigus vulgaris (PV), one with paraneoplastic pemphigus (PP), and three with pemphigus erythematosus (PE). Frozen epidermal sheets, separated from the dermis with a scalpel, were used as a substrate. Diagnosis with routine fluorescein isothiocyanate (FITC) antibodies was made. Results: In all patients a pericellular deposition of IgG was evident and in eight of these patients a pericellular deposition of C3 was present. In two cases of PE and one of PP, the C3 deposits were also present in the lower part of basal keratinocytes. Conclusion: This diagnostic method without skin biopsy is easy to perform and, together with the histology and clinical aspects, could be a useful tool in the diagnosis of pemphigus. We recommend this method when the patient is allergic to local anaesthetics, the patient easily produces hypertrophic scars, or in follow-up of already biopsied patients.
1998
Direct immunofluorescence diagnosis of pemphigus without biopsy / Feliciani, Claudio; b and Mohammad Pour, C. a.; and Toto, S. a.; and Coscione, P. a.; and Amerio, G. a.; and Amerio, P. a.; P, Asha. - In: JOURNAL OF CUTANEOUS MEDICINE AND SURGERY. - ISSN 1203-4754. - 2:4(1998), pp. 209-211.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2862906
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