Introduction: Leishmania infantum infects dogs in several Mediterranean countries, including Italy. It is known, however, that many infected dogs will not develop clinical manifestations of canine leishmaniosis (CL). Several studies have been aimed at identifying possible breed predisposition to disease and the Boxer breed, among others, has been reported to be more predisposed. The aim of the present study was to analyze peripheral blood lymphocyte subset populations in healthy boxers and in boxers affected by leishmaniosis. Materials and Methods: Nine healthy, L. infantum-negative boxers and 6 boxers with CL were studied. Infection status was determined by antibody titre and PCR. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient and analyzed in flow cytometry for the expression of: CD3, CD4,CD8, CD21, CD11a/CD18, CD25 and Foxp3. Double staining was carried out for CD4/CD8 and CD4/FoxP3, while triple staining was carried out for CD4/CD25/FoxP3. Results were analyzed with the Kolmogorov-Smirnov test, with significant differences between healthy and sick dogs for each subset at p ≤ 0.05. Results: No significant differences were observed between sick dogs and healthy dogs for CD3+, CD4+CD8- or CD4+/Foxp3+ cells; levels of CD4-/CD8+ and CD4+/CD8+ lymphocytes tended to be higher in dogs with CL, even though this was not significant. The levels of CD4+/CD25+/FoxP3+ were extremely low in both groups. Conclusions: Further studies with a larger population of animals are necessary to confirm or exclude a role for lymphocyte subset variation in the development of CL in predisposed breeds.

BLOOD LYMPHOCYTE SUBSET POPULATIONS IN HEALTHY BOXERS AND IN BOXERS AFFECTED BY CANINE LEISHMANIOSIS (L. INFANTUM) / Passeri, Benedetta; Mangia, Carlo; DE ANGELIS, Elena; Aliani, Debora; Maria Cantoni, Anna; Melis, Gabriele Costantino; Kramer, Laura Helen. - (2017). ((Intervento presentato al convegno 3nd Joint European Congress of the ESVP, ESTP and ECVP tenutosi a Lyon, France nel 30th August – 2nd September 2017.

BLOOD LYMPHOCYTE SUBSET POPULATIONS IN HEALTHY BOXERS AND IN BOXERS AFFECTED BY CANINE LEISHMANIOSIS (L. INFANTUM)

Benedetta Passeri
;
Carlo Mangia;Elena De Angelis;Gabriele Costantino Melis;Laura Helen Kramer
2017

Abstract

Introduction: Leishmania infantum infects dogs in several Mediterranean countries, including Italy. It is known, however, that many infected dogs will not develop clinical manifestations of canine leishmaniosis (CL). Several studies have been aimed at identifying possible breed predisposition to disease and the Boxer breed, among others, has been reported to be more predisposed. The aim of the present study was to analyze peripheral blood lymphocyte subset populations in healthy boxers and in boxers affected by leishmaniosis. Materials and Methods: Nine healthy, L. infantum-negative boxers and 6 boxers with CL were studied. Infection status was determined by antibody titre and PCR. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient and analyzed in flow cytometry for the expression of: CD3, CD4,CD8, CD21, CD11a/CD18, CD25 and Foxp3. Double staining was carried out for CD4/CD8 and CD4/FoxP3, while triple staining was carried out for CD4/CD25/FoxP3. Results were analyzed with the Kolmogorov-Smirnov test, with significant differences between healthy and sick dogs for each subset at p ≤ 0.05. Results: No significant differences were observed between sick dogs and healthy dogs for CD3+, CD4+CD8- or CD4+/Foxp3+ cells; levels of CD4-/CD8+ and CD4+/CD8+ lymphocytes tended to be higher in dogs with CL, even though this was not significant. The levels of CD4+/CD25+/FoxP3+ were extremely low in both groups. Conclusions: Further studies with a larger population of animals are necessary to confirm or exclude a role for lymphocyte subset variation in the development of CL in predisposed breeds.
BLOOD LYMPHOCYTE SUBSET POPULATIONS IN HEALTHY BOXERS AND IN BOXERS AFFECTED BY CANINE LEISHMANIOSIS (L. INFANTUM) / Passeri, Benedetta; Mangia, Carlo; DE ANGELIS, Elena; Aliani, Debora; Maria Cantoni, Anna; Melis, Gabriele Costantino; Kramer, Laura Helen. - (2017). ((Intervento presentato al convegno 3nd Joint European Congress of the ESVP, ESTP and ECVP tenutosi a Lyon, France nel 30th August – 2nd September 2017.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2854771
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