Reliable chemical sensors with high selectivity and sensitivity toward specific target molecules require rational synthesis of receptors, in-depth characterization of their complexation abilities and highly efficient transduction of the molecular recognition event. Here we report a steady-state and time resolved fluorescence investigation of EtQxBox, a fluorescent conformationally blocked quinoxaline based cavitand, aimed at assessing its selectivity toward aromatic versus non-aromatic analytes in solution. Fluorescence quenching of the EtQxBox in acetone is observed at increasing concentration of both aromatic (i.e. benzonitrile) and aliphatic (i.e. acetonitrile) compounds. The combination with fluorescence lifetime measurements permits to discriminate the predominantly static quenching of the aromatic analyte, due to non-fluorescent host–guest complex formation, from the mostly dynamic quenching of the non-aromatic compound, resulting from aspecific diffusive collisions between the fluorophore and the quencher. The equilibrium association constants for both the complexes have been estimated using Stern–Volmer model.

Assessment of EtQxBox complexation in solution by steady-state and time-resolved fluorescence spectroscopy / Aprile, Arianna; Palermo, Giovanna; De Luca, Antonio; Pinalli, Roberta; Dalcanale, Enrico; Pagliusi, Pasquale. - In: RSC ADVANCES. - ISSN 2046-2069. - 8:29(2018), pp. 16314-16318. [10.1039/C8RA02875C]

Assessment of EtQxBox complexation in solution by steady-state and time-resolved fluorescence spectroscopy

Pinalli, Roberta;Dalcanale, Enrico;
2018

Abstract

Reliable chemical sensors with high selectivity and sensitivity toward specific target molecules require rational synthesis of receptors, in-depth characterization of their complexation abilities and highly efficient transduction of the molecular recognition event. Here we report a steady-state and time resolved fluorescence investigation of EtQxBox, a fluorescent conformationally blocked quinoxaline based cavitand, aimed at assessing its selectivity toward aromatic versus non-aromatic analytes in solution. Fluorescence quenching of the EtQxBox in acetone is observed at increasing concentration of both aromatic (i.e. benzonitrile) and aliphatic (i.e. acetonitrile) compounds. The combination with fluorescence lifetime measurements permits to discriminate the predominantly static quenching of the aromatic analyte, due to non-fluorescent host–guest complex formation, from the mostly dynamic quenching of the non-aromatic compound, resulting from aspecific diffusive collisions between the fluorophore and the quencher. The equilibrium association constants for both the complexes have been estimated using Stern–Volmer model.
Assessment of EtQxBox complexation in solution by steady-state and time-resolved fluorescence spectroscopy / Aprile, Arianna; Palermo, Giovanna; De Luca, Antonio; Pinalli, Roberta; Dalcanale, Enrico; Pagliusi, Pasquale. - In: RSC ADVANCES. - ISSN 2046-2069. - 8:29(2018), pp. 16314-16318. [10.1039/C8RA02875C]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2846842
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