Kisspeptin and its receptor KISS1R are involved in the neuroendocrine regulation of mammalian reproduction and their role in the regulation of follicular development can be hypothesized. The present work was designed to confirm the immunopresence of kisspeptin and its receptor in the swine ovary and to study the possible effects of kisspeptin 10 and its antagonist, kisspeptin 234, on granulosa cells main functional parameters, namely cell proliferation, steroid production and redox status. The potential modulating action of the two molecules on angiogenesis was also investigated by means of an angiogenesis bioassay. The immunopresence of kisspeptin and its receptor KISS1R in a swine granulosa cell model was confirmed by our data. Kisspeptin 10 stimulating effect on progesterone production would suggest its possible role in the regulation of luteinization of granulosa cells. We can also hypothesize kisspeptin 10 involvement in the control of the ovarian follicle antioxidant defense system, given that it displays potentiating effects on non-enzymatic scavenging activity. In addition, our results suggest that this molecule could play a significant role in the physiological development of new ovarian vessels. Additional studies are needed in order to confirm the functional significance of the kisspeptin/ KISS1R system within the swine ovary
Presence and function of kisspeptin/KISS1R system in swine ovarian follicles / Basini, G.; Grasselli, F.; Bussolati, S.; Ciccimarra, R.; Maranesi, M.; Bufalari, A.; Parillo, F.; Zerani ., M.. - In: THERIOGENOLOGY. - ISSN 1879-3231. - 115:(2018), pp. 1-8. [10.1016/j.theriogenology.2018.04.006]
Presence and function of kisspeptin/KISS1R system in swine ovarian follicles
G. Basini;F. Grasselli;S. Bussolati;R. Ciccimarra;
2018-01-01
Abstract
Kisspeptin and its receptor KISS1R are involved in the neuroendocrine regulation of mammalian reproduction and their role in the regulation of follicular development can be hypothesized. The present work was designed to confirm the immunopresence of kisspeptin and its receptor in the swine ovary and to study the possible effects of kisspeptin 10 and its antagonist, kisspeptin 234, on granulosa cells main functional parameters, namely cell proliferation, steroid production and redox status. The potential modulating action of the two molecules on angiogenesis was also investigated by means of an angiogenesis bioassay. The immunopresence of kisspeptin and its receptor KISS1R in a swine granulosa cell model was confirmed by our data. Kisspeptin 10 stimulating effect on progesterone production would suggest its possible role in the regulation of luteinization of granulosa cells. We can also hypothesize kisspeptin 10 involvement in the control of the ovarian follicle antioxidant defense system, given that it displays potentiating effects on non-enzymatic scavenging activity. In addition, our results suggest that this molecule could play a significant role in the physiological development of new ovarian vessels. Additional studies are needed in order to confirm the functional significance of the kisspeptin/ KISS1R system within the swine ovaryI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
