The isolation and characterization from the sand fly Phlebotomus perniciosus of a Wickerhamomyces anomalus yeast strain (Wa1F1) displaying the killer phenotype was recently reported. In the present work, the killer toxin (KT) produced by Wa1F1 was purified and characterized, and its antimicrobial activity in vitro was investigated against fluconazole- susceptible and -resistant clinical isolates and laboratory strains of Candida albicans and C. glabrata displaying known mutations. Wa1F1-KT showed a differential killing ability against different mutant strains of the same species. The results may be useful for the design of therapeutic molecules based on Wa1F1-KT and the study of yeast resistance mechanisms.

Candidacidal activity of a novel killer toxin from Wickerhamomyces anomalus against fluconazole-susceptible and -resistant strains / Giovati, Laura; Santinoli, Claudia; Ferrari, Elena; Ciociola, Tecla; Martin, Elena; Bandi, Claudio; Ricci, Irene; Epis, Sara; Conti, Stefania. - In: TOXINS. - ISSN 2072-6651. - 10:2(2018), p. 68. [10.3390/toxins10020068]

Candidacidal activity of a novel killer toxin from Wickerhamomyces anomalus against fluconazole-susceptible and -resistant strains

Giovati, Laura;Santinoli, Claudia;Ferrari, Elena;Ciociola, Tecla;Conti, Stefania
2018

Abstract

The isolation and characterization from the sand fly Phlebotomus perniciosus of a Wickerhamomyces anomalus yeast strain (Wa1F1) displaying the killer phenotype was recently reported. In the present work, the killer toxin (KT) produced by Wa1F1 was purified and characterized, and its antimicrobial activity in vitro was investigated against fluconazole- susceptible and -resistant clinical isolates and laboratory strains of Candida albicans and C. glabrata displaying known mutations. Wa1F1-KT showed a differential killing ability against different mutant strains of the same species. The results may be useful for the design of therapeutic molecules based on Wa1F1-KT and the study of yeast resistance mechanisms.
Candidacidal activity of a novel killer toxin from Wickerhamomyces anomalus against fluconazole-susceptible and -resistant strains / Giovati, Laura; Santinoli, Claudia; Ferrari, Elena; Ciociola, Tecla; Martin, Elena; Bandi, Claudio; Ricci, Irene; Epis, Sara; Conti, Stefania. - In: TOXINS. - ISSN 2072-6651. - 10:2(2018), p. 68. [10.3390/toxins10020068]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2838232
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 6
social impact