Fusariumverticillioidescausesearrotdiseaseinmaizeanditscontaminationwithfumonisins, mycotoxinsharmfulforhumansandlivestock. Lipids,andtheiroxidizedforms,maydrivethefateof thisdisease. Inapreviousstudy,wehaveexploredtheroleofoxylipinsinthisinteractionbydeleting by standard transformation procedures a linoleate diol synthase-coding gene, lds1, in F. verticillioides. A profound phenotypic diversity in the mutants generated has prompted us to investigate more deeply the whole genome of two lds1-deleted strains. Bioinformatics analyses pinpoint significant differencesinthegenomesequencesemergedbetweenthewildtypeandthelds1-mutantsfurtherthan those trivially attributable to the deletion of the lds1 locus, such as single nucleotide polymorphisms, small deletion/insertion polymorphisms and structural variations. Results suggest that the effect of a (theoretically) punctual transformation event might have enhanced the natural mechanisms of genomicvariabilityandthattransformationpractices,commonlyusedinthereversegeneticsoffungi, may potentially be responsible for unexpected, stochastic and henceforth off-target rearrangements throughout the genome.
Careful with that axe, gene, genome perturbation after a PEG-mediated protoplast transformation in Fusarium verticillioides / Scala, V.; Grottoli, A.; Cigliano, R. A.; Anzar, I.; Beccaccioli, M.; Fanelli, C.; Dall’Asta, C.; Battilani, P.; Reverberi, Maddalena; Sanseverino, W.. - In: TOXINS. - ISSN 2072-6651. - 9:6(2017). [10.3390/toxins9060183]
Careful with that axe, gene, genome perturbation after a PEG-mediated protoplast transformation in Fusarium verticillioides
Dall’asta C.;REVERBERI, MADDALENA;
2017-01-01
Abstract
Fusariumverticillioidescausesearrotdiseaseinmaizeanditscontaminationwithfumonisins, mycotoxinsharmfulforhumansandlivestock. Lipids,andtheiroxidizedforms,maydrivethefateof thisdisease. Inapreviousstudy,wehaveexploredtheroleofoxylipinsinthisinteractionbydeleting by standard transformation procedures a linoleate diol synthase-coding gene, lds1, in F. verticillioides. A profound phenotypic diversity in the mutants generated has prompted us to investigate more deeply the whole genome of two lds1-deleted strains. Bioinformatics analyses pinpoint significant differencesinthegenomesequencesemergedbetweenthewildtypeandthelds1-mutantsfurtherthan those trivially attributable to the deletion of the lds1 locus, such as single nucleotide polymorphisms, small deletion/insertion polymorphisms and structural variations. Results suggest that the effect of a (theoretically) punctual transformation event might have enhanced the natural mechanisms of genomicvariabilityandthattransformationpractices,commonlyusedinthereversegeneticsoffungi, may potentially be responsible for unexpected, stochastic and henceforth off-target rearrangements throughout the genome.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
