Introduction: Inhibin-α is a protein produced by gonadal stromal cells responsible for the physiological regulation of the productive axis in both genders, and is connected to mechanisms of cell differentiation and tumor suppression. GATA-4 is a transcription factor which is differentially expressed depending on gonadal developmental stages and pathological conditions. Materials and Methods: Immunohistochemistry for Inhibin-α and GATA-4 was performed on normal gonads of both genders at different developmental stages and also on neoplasms. Immunostaining was evaluated with a semiquantitative score. Results: Inhibin-α was expressed in Sertoli cells of normal testis of prepubertal dogs. Meanwhile in mature dogs, Leydig cells were also positive. In both sex-cord stromal tumors (Leydig and Sertoli cell tumors) neoplastic cells were positive. In normal ovary, follicular epithelial cells of primordial follicles and granulosa cells of remnant follicle stages were positive. Granulosa cell tumors expressed Inhibin-α in neoplastic cells, meanwhile papillary cystadenomas were negative. GATA-4 was expressed in normal testis of prepubertal and pubertal dogs in Sertoli and Leydig cells. In both sex-cord stromal tumors, neoplastic cells were positive. In normal ovary, prepubertal dogs had positive stromal and epithelial cells. In pubertal and mature females, granulosa cells were positive with viable expression in stromal cells. Granulosa cell tumors expressed GATA-4 in neoplastic cells and papillary cystadenoma was negative. Conclusions: Inhibin-α can be used as marker of normal and neoplastic sex-cord stromal cells in testis and ovarian granulosa cells. GATA-4 has a similar expression in testis and a constant expression in normal and neoplastic ovarian granulosa cells.

PRELIMINARY EVALUATION OF IMMUNOHISTOCHEMICAL EXPRESSION OF INHIBIN-α AND GATA-4 IN NORMAL AND NEOPLASTIC CANINE GONADS / Bertani, Valeria; DI LECCE, Rosanna; Rossi, C; Corradi, Attilio; Cantoni, Anna Maria. - STAMPA. - 158(2018), pp. 124-124. ((Intervento presentato al convegno 3rd Joint European Congress of the ESVP, ESTP and ECVP tenutosi a Lione nel 30th August - 2nd September 2017.

PRELIMINARY EVALUATION OF IMMUNOHISTOCHEMICAL EXPRESSION OF INHIBIN-α AND GATA-4 IN NORMAL AND NEOPLASTIC CANINE GONADS

BERTANI, Valeria;DI LECCE, Rosanna;CORRADI, Attilio;CANTONI, Anna Maria
2018

Abstract

Introduction: Inhibin-α is a protein produced by gonadal stromal cells responsible for the physiological regulation of the productive axis in both genders, and is connected to mechanisms of cell differentiation and tumor suppression. GATA-4 is a transcription factor which is differentially expressed depending on gonadal developmental stages and pathological conditions. Materials and Methods: Immunohistochemistry for Inhibin-α and GATA-4 was performed on normal gonads of both genders at different developmental stages and also on neoplasms. Immunostaining was evaluated with a semiquantitative score. Results: Inhibin-α was expressed in Sertoli cells of normal testis of prepubertal dogs. Meanwhile in mature dogs, Leydig cells were also positive. In both sex-cord stromal tumors (Leydig and Sertoli cell tumors) neoplastic cells were positive. In normal ovary, follicular epithelial cells of primordial follicles and granulosa cells of remnant follicle stages were positive. Granulosa cell tumors expressed Inhibin-α in neoplastic cells, meanwhile papillary cystadenomas were negative. GATA-4 was expressed in normal testis of prepubertal and pubertal dogs in Sertoli and Leydig cells. In both sex-cord stromal tumors, neoplastic cells were positive. In normal ovary, prepubertal dogs had positive stromal and epithelial cells. In pubertal and mature females, granulosa cells were positive with viable expression in stromal cells. Granulosa cell tumors expressed GATA-4 in neoplastic cells and papillary cystadenoma was negative. Conclusions: Inhibin-α can be used as marker of normal and neoplastic sex-cord stromal cells in testis and ovarian granulosa cells. GATA-4 has a similar expression in testis and a constant expression in normal and neoplastic ovarian granulosa cells.
PRELIMINARY EVALUATION OF IMMUNOHISTOCHEMICAL EXPRESSION OF INHIBIN-α AND GATA-4 IN NORMAL AND NEOPLASTIC CANINE GONADS / Bertani, Valeria; DI LECCE, Rosanna; Rossi, C; Corradi, Attilio; Cantoni, Anna Maria. - STAMPA. - 158(2018), pp. 124-124. ((Intervento presentato al convegno 3rd Joint European Congress of the ESVP, ESTP and ECVP tenutosi a Lione nel 30th August - 2nd September 2017.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2830554
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