Androgen hormones play a significant role in regulating bone morphogenesis and in maintaining bone homeostasis throughout life. This study aimed to investigate the local effects of the non-aromatizable androgen stanozolol (ST) on bone regeneration in rats. Bilateral critical-size defects were created in the parietal bone of 26 male Wistar rats: the defect on one side was filled with a deproteinized bovine bone scaffold (DBB) soaked in ST solution (test) and the contralateral with DBB alone (control). Samples were collected at one month and three months. Histomorphometry revealed a significantly higher new bone formation (NB) (24.41% ± 4.14% versus 15.01% ± 2.43%, p < 0.05) and mineral apposition rate (MAR) (9.20 μm/day ± 0.37 versus 6.50 μm/day ± 1.09, p < 0.05) in the test versus control group at one month. Accordingly, real time-polymerase chain reaction revealed a consistently higher Runx2 expression in test samples (fold change test/control: 4.50 ± 1.17, p ≤ 0.05). No morphometrical differences between groups were detected at three months (p > 0.05). However, test samples were characterized by an increase in blood capillary density from one month (11.43 n mm-2 ± 2.01) to three months (28.26 n mm-2 ± 5.62), providing evidence of a vital remodeling tissue. Control samples presented a decrease of anti-Osterix (SP7)/anti-osteocalcin (BGLAP) (3.9 n mm-2 ± 0.32 versus 1.01 n mm-2 ± 0.20) and alkaline phosphatase (ALP) (12.14 n mm-2 ± 6.29 versus 6.29 n mm-2 ± 2.73) immunohistochemical-positive elements, which was suggestive of a stabilized healing phase. Based on these observations, local ST administration boosted bone regeneration in rat calvarial critical-size defects at one month. This study showed the potential of local steroid delivery in bone regeneration.

Stanozolol-soaked grafts enhance new bone formation in rat calvarial critical-size defects / Ghiacci, Giulia; Graiani, Gallia; Cacchioli, Antonio; Galli, Carlo; Lumetti, Simone; Ravanetti, Francesca; Elviri, Lisa; Manfredi, Edoardo; Macaluso, G. M.; Sala, Roberto. - In: BIOMEDICAL MATERIALS. - ISSN 1748-6041. - 12:4(2017), p. 045016. [10.1088/1748-605X/aa71bc]

Stanozolol-soaked grafts enhance new bone formation in rat calvarial critical-size defects

GHIACCI, GIULIA;GRAIANI, Gallia;CACCHIOLI, Antonio;GALLI, CARLO;LUMETTI, Simone;RAVANETTI, Francesca;ELVIRI, Lisa;MANFREDI, Edoardo;Macaluso, G. M.;SALA, Roberto
Writing – Review & Editing
2017

Abstract

Androgen hormones play a significant role in regulating bone morphogenesis and in maintaining bone homeostasis throughout life. This study aimed to investigate the local effects of the non-aromatizable androgen stanozolol (ST) on bone regeneration in rats. Bilateral critical-size defects were created in the parietal bone of 26 male Wistar rats: the defect on one side was filled with a deproteinized bovine bone scaffold (DBB) soaked in ST solution (test) and the contralateral with DBB alone (control). Samples were collected at one month and three months. Histomorphometry revealed a significantly higher new bone formation (NB) (24.41% ± 4.14% versus 15.01% ± 2.43%, p < 0.05) and mineral apposition rate (MAR) (9.20 μm/day ± 0.37 versus 6.50 μm/day ± 1.09, p < 0.05) in the test versus control group at one month. Accordingly, real time-polymerase chain reaction revealed a consistently higher Runx2 expression in test samples (fold change test/control: 4.50 ± 1.17, p ≤ 0.05). No morphometrical differences between groups were detected at three months (p > 0.05). However, test samples were characterized by an increase in blood capillary density from one month (11.43 n mm-2 ± 2.01) to three months (28.26 n mm-2 ± 5.62), providing evidence of a vital remodeling tissue. Control samples presented a decrease of anti-Osterix (SP7)/anti-osteocalcin (BGLAP) (3.9 n mm-2 ± 0.32 versus 1.01 n mm-2 ± 0.20) and alkaline phosphatase (ALP) (12.14 n mm-2 ± 6.29 versus 6.29 n mm-2 ± 2.73) immunohistochemical-positive elements, which was suggestive of a stabilized healing phase. Based on these observations, local ST administration boosted bone regeneration in rat calvarial critical-size defects at one month. This study showed the potential of local steroid delivery in bone regeneration.
Stanozolol-soaked grafts enhance new bone formation in rat calvarial critical-size defects / Ghiacci, Giulia; Graiani, Gallia; Cacchioli, Antonio; Galli, Carlo; Lumetti, Simone; Ravanetti, Francesca; Elviri, Lisa; Manfredi, Edoardo; Macaluso, G. M.; Sala, Roberto. - In: BIOMEDICAL MATERIALS. - ISSN 1748-6041. - 12:4(2017), p. 045016. [10.1088/1748-605X/aa71bc]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2830084
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