We previously showed that Irisin, a myokine released from skeletal muscle after physical exercise, plays a central role in the control of bone mass. Here we report that treatment with recombinant Irisin prevented bone loss in hind-limb suspended mice when administered during suspension (preventive protocol) and induced recovery of bone mass when mice were injected after bone loss due to a suspension period of 4 weeks (curative protocol). MicroCT analysis of femurs showed that r-Irisin preserved both cortical and trabecular bone mineral density, and prevented a dramatic decrease of the trabecular bone volume fraction. Moreover, r-Irisin protected against muscle mass decline in the hind-limb suspended mice, and maintained the fiber cross-sectional area. Notably, the decrease of myosin type II expression in unloaded mice was completely prevented by r-Irisin administration. Our data reveal for the first time that Irisin retrieves disuseâ € induced bone loss and muscle atrophy. These findings may lead to development of an Irisin-based therapy for elderly immobile osteoporotic and physically disable patients, and might represent a countermeasure for astronauts subjected to microgravity-induced bone and muscle losses.

Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice / Colaianni, Graziana; Mongelli, Teresa; Cuscito, Concetta; Pignataro, Paolo; Lippo, Luciana; Spiro, Giovanna; Notarnicola, Angela; Severi, Ilenia; Passeri, Giovanni; Mori, Giorgio; Brunetti, Giacomina; Moretti, Biagio; Tarantino, Umberto; Colucci, Silvia C.; Reseland, Janne E.; Vettor, Roberto; Cinti, Saverio; Grano, Maria. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7:1(2017), p. 2811. [10.1038/s41598-017-02557-8]

Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice

PASSERI, Giovanni;
2017-01-01

Abstract

We previously showed that Irisin, a myokine released from skeletal muscle after physical exercise, plays a central role in the control of bone mass. Here we report that treatment with recombinant Irisin prevented bone loss in hind-limb suspended mice when administered during suspension (preventive protocol) and induced recovery of bone mass when mice were injected after bone loss due to a suspension period of 4 weeks (curative protocol). MicroCT analysis of femurs showed that r-Irisin preserved both cortical and trabecular bone mineral density, and prevented a dramatic decrease of the trabecular bone volume fraction. Moreover, r-Irisin protected against muscle mass decline in the hind-limb suspended mice, and maintained the fiber cross-sectional area. Notably, the decrease of myosin type II expression in unloaded mice was completely prevented by r-Irisin administration. Our data reveal for the first time that Irisin retrieves disuseâ € induced bone loss and muscle atrophy. These findings may lead to development of an Irisin-based therapy for elderly immobile osteoporotic and physically disable patients, and might represent a countermeasure for astronauts subjected to microgravity-induced bone and muscle losses.
2017
Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice / Colaianni, Graziana; Mongelli, Teresa; Cuscito, Concetta; Pignataro, Paolo; Lippo, Luciana; Spiro, Giovanna; Notarnicola, Angela; Severi, Ilenia; Passeri, Giovanni; Mori, Giorgio; Brunetti, Giacomina; Moretti, Biagio; Tarantino, Umberto; Colucci, Silvia C.; Reseland, Janne E.; Vettor, Roberto; Cinti, Saverio; Grano, Maria. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7:1(2017), p. 2811. [10.1038/s41598-017-02557-8]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2828068
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