4.4 Expression of Orexin A and its receptors in the porcine ovary Ragionieri L., Ravanetti F., Botti M., Ciccimarra R., Bussolati S., Basini G., Gazza F., and Cacchioli A. Department of Veterinary Sciences, University of Parma. Orexin A (OXA), and B are two neuropeptides mainly synthesized in the lateral hypothalamus, from a common precursor called prepro-orexin and involved in different functions such as regulation of food intake, energy balance, reproductive activities, sleep and wakefulness. Their action is mediated by binding G-protein-coupled receptors, termed orexin receptor 1 (OXR1) and orexin receptor 2 (OXR2). As orexins and their receptors were previously described also in different tissues and organs outside the brain, we investigated their presence in the porcine ovary. Using double labelling immunofluorescence techniques, we observed OXA and its receptors in granulosa, thecal and luteal cells, moreover in corpora albicantia, atretic follicles and interstitial cells of the porcine ovary. These last three localizations had never been investigated in previous research. OXA and OXR2 appeared more diffused in the cytoplasm and OXR1 associated to the nuclear envelop of the granulosa cells, while in thecal, luteal and interstitial cells, all the substances appeared associated to cytoplasmic vesicles. We also proved, by real-time Polymerase-Chain-Reaction, that granulosa cells of large follicles and luteal cells express the prepro-orexin gene. In vitro administration of OXA stimulated the growth and induced an increased production of oestradiol in granulosa cells. The progesterone production was instead not affected in granulosa cells, while inhibited in luteal cells. Taken together these findings demonstrate that porcine ovary contains different cells types able to secrete and/or internalize OXA, thus suggesting both autocrine and paracrine modalities of action of OXA. Moreover, OXA could be involved in modulating ovarian steroidogenesis and follicle growth.
Expression of Orexin A and its receptors in the porcine ovary / Ragionieri, Luisa; Ravanetti, Francesca; Botti, Maddalena; Ciccimarra, Roberta; Bussolati, Simona; Basini, Giuseppina; Gazza, Ferdinando; Cacchioli, Antonio. - ELETTRONICO. - (2017), pp. 13-13. (Intervento presentato al convegno XI Congresso Nazionale Associazione Morfologi Veterinari tenutosi a ROMA - Palazzina dell'Auditorio - Via della Lungara 230 nel 25-26 Maggio 2017).
Expression of Orexin A and its receptors in the porcine ovary
RAGIONIERI, Luisa;RAVANETTI, Francesca;BOTTI, Maddalena;CICCIMARRA, ROBERTA;BUSSOLATI, Simona;BASINI, Giuseppina;GAZZA, Ferdinando;CACCHIOLI, Antonio
2017-01-01
Abstract
4.4 Expression of Orexin A and its receptors in the porcine ovary Ragionieri L., Ravanetti F., Botti M., Ciccimarra R., Bussolati S., Basini G., Gazza F., and Cacchioli A. Department of Veterinary Sciences, University of Parma. Orexin A (OXA), and B are two neuropeptides mainly synthesized in the lateral hypothalamus, from a common precursor called prepro-orexin and involved in different functions such as regulation of food intake, energy balance, reproductive activities, sleep and wakefulness. Their action is mediated by binding G-protein-coupled receptors, termed orexin receptor 1 (OXR1) and orexin receptor 2 (OXR2). As orexins and their receptors were previously described also in different tissues and organs outside the brain, we investigated their presence in the porcine ovary. Using double labelling immunofluorescence techniques, we observed OXA and its receptors in granulosa, thecal and luteal cells, moreover in corpora albicantia, atretic follicles and interstitial cells of the porcine ovary. These last three localizations had never been investigated in previous research. OXA and OXR2 appeared more diffused in the cytoplasm and OXR1 associated to the nuclear envelop of the granulosa cells, while in thecal, luteal and interstitial cells, all the substances appeared associated to cytoplasmic vesicles. We also proved, by real-time Polymerase-Chain-Reaction, that granulosa cells of large follicles and luteal cells express the prepro-orexin gene. In vitro administration of OXA stimulated the growth and induced an increased production of oestradiol in granulosa cells. The progesterone production was instead not affected in granulosa cells, while inhibited in luteal cells. Taken together these findings demonstrate that porcine ovary contains different cells types able to secrete and/or internalize OXA, thus suggesting both autocrine and paracrine modalities of action of OXA. Moreover, OXA could be involved in modulating ovarian steroidogenesis and follicle growth.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
