Bio-functionalization of inner surfaces of all silica Hollow Core-Photonic Crystal Fibers (HC-PCF) has been investigated. The approach is based on layer-by-layer self-assembly Peptide Nucleic Acid (PNA) probes, which is an oligonucleotide mimic that is well suited for specific DNA target recognition. Two kinds of HC-PCFs have been considered: a photonic Bragg fiber and a hollow core (HC-1060) fiber. After spectral characterization and internal surface functionalization by using PNA probes, genomic DNA solutions from soy flour were infiltrated into the fibers. The experimental results indicate that hybridization of the complementary strand of target DNA increases the thickness of the silica layer and leads up to the generation of surface modes, resulting in a significant modulation of the transmission spectra. Numerical analysis confirms such behavior, suggesting the possibility to realize biological sensing. © 2014 SPIE.
Bio-functionalized hollow core photonic crystal fibers for label-free DNA detection / Candiani, Alessandro; Salloom, Hussein T.; Coscelli, Enrico; Sozzi, Michele; Manicardi, Alex; Ahmad, Ahmad K.; Al Janabi, A. Hadi; Corradini, Roberto; Picchi, Giorgio; Cucinotta, Annamaria; Selleri, Stefano. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - 8938:(2014), p. 89380T. (Intervento presentato al convegno Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV tenutosi a San Francisco, CA, usa nel 2014) [10.1117/12.2039665].
Bio-functionalized hollow core photonic crystal fibers for label-free DNA detection
CANDIANI, Alessandro;COSCELLI, Enrico;SOZZI, MICHELE;MANICARDI, Alex;CORRADINI, Roberto;PICCHI, Giorgio;CUCINOTTA, Annamaria;SELLERI, Stefano
2014-01-01
Abstract
Bio-functionalization of inner surfaces of all silica Hollow Core-Photonic Crystal Fibers (HC-PCF) has been investigated. The approach is based on layer-by-layer self-assembly Peptide Nucleic Acid (PNA) probes, which is an oligonucleotide mimic that is well suited for specific DNA target recognition. Two kinds of HC-PCFs have been considered: a photonic Bragg fiber and a hollow core (HC-1060) fiber. After spectral characterization and internal surface functionalization by using PNA probes, genomic DNA solutions from soy flour were infiltrated into the fibers. The experimental results indicate that hybridization of the complementary strand of target DNA increases the thickness of the silica layer and leads up to the generation of surface modes, resulting in a significant modulation of the transmission spectra. Numerical analysis confirms such behavior, suggesting the possibility to realize biological sensing. © 2014 SPIE.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
