BACKGROUND: Despite continuous research efforts, bacterio(phages) infecting Lactococcus lactis starter strains persist as a major threat to dairy fermentations. The lactococcal P335 phages, which are currently classified into four sub-groups (I-IV), are the second most frequently isolated phage group in an industrial dairy context. RESULTS: The current work describes the isolation and comparative genomic analysis of 17 novel P335 group phages. Detailed analysis of the genomic region of P335 phages encoding the so-called "baseplate", which includes the receptor binding protein (RBP) was combined with a functional characterization of the RBP of sub-group III and IV phages. Additionally, calcium-dependence assays revealed a specific requirement for calcium by sub-group IV phages while host range analysis highlighted a higher number of strains with CWPS type A (11 of 39 strains) are infected by the P335 phages assessed in this study than those with a C (five strains), B (three of 39 strains) or unknown (one of 39 strains) CWPS type. CONCLUSIONS: These analyses revealed significant divergence among RBP sequences, apparently reflecting their unique interactions with the host and particularly for strains with a type A CWPS. The implications of the genomic architecture of lactococcal P335 phages on serving as a general model for Siphoviridae phages are discussed.
Genetic and functional characterisation of the lactococcal P335 phage-host interactions / Mahony, J; Oliveira, J; Collins, B; Hanemaaijer, L; Lugli, Ga; Neve, H; Ventura, M; Kouwen, Tr; Cambillau, C; van Sinderen, D;. - In: BMC GENOMICS. - ISSN 1471-2164. - 18:1(2017), pp. 1-16. [10.1186/s12864-017-3537-5]
|Appare nelle tipologie:||1.1 Articolo su rivista|