Aggregation is a major problem for anti-microbial phodynamic applications of hydrophobic photosensitizers since it strongly reduces the amount of singlet oxygen generated in aqueous solutions. Binding of hypericin (Hyp) to the milk whey protein β-lactoglobulin (βLG), occurring at the two hydrophobic cavities located at the interface of the protein homodimer, can be exploited to confer water-solubility and biocompatibility to the photosensitizer. The introduction of a small amount of the organic cosolvent dimethyl sulfoxide (DMSO) leads to a remarkable improvement of the photophysical properties of the complex Hyp-βLG by increasing its fluorescence emission and singlet oxygen photosensitization quantum yields. Surprisingly, the ability of the complex to photo-inactivate bacteria of the strain Staphylococcus aureus is strongly reduced in the presence of DMSO, despite the higher yield of photosensitization. The reasons for this apparently contradictory behavior are investigated, providing new insights about the use of carrier systems for hydrophobic photosensitizers.

Tuning the local solvent composition at a drug carrier surface: Effect of dimethyl sulfoxide/water mixture on the photofunctional properties of hypericin--lactoglobulin complexes / Delcanale, Pietro; Rodríguez-Amigo, Beatriz; Juárez-Jiménez, Jordi; Luque, F. Javier; Abbruzzetti, Stefania; Agut, Montserrat; Nonell, Santi; Viappiani, Cristiano. - In: JOURNAL OF MATERIALS CHEMISTRY. B. - ISSN 2050-750X. - 5(2017), pp. 1633-1641. [10.1039/C7TB00081B]

Tuning the local solvent composition at a drug carrier surface: Effect of dimethyl sulfoxide/water mixture on the photofunctional properties of hypericin--lactoglobulin complexes

DELCANALE, Pietro;ABBRUZZETTI, Stefania;VIAPPIANI, Cristiano
2017

Abstract

Aggregation is a major problem for anti-microbial phodynamic applications of hydrophobic photosensitizers since it strongly reduces the amount of singlet oxygen generated in aqueous solutions. Binding of hypericin (Hyp) to the milk whey protein β-lactoglobulin (βLG), occurring at the two hydrophobic cavities located at the interface of the protein homodimer, can be exploited to confer water-solubility and biocompatibility to the photosensitizer. The introduction of a small amount of the organic cosolvent dimethyl sulfoxide (DMSO) leads to a remarkable improvement of the photophysical properties of the complex Hyp-βLG by increasing its fluorescence emission and singlet oxygen photosensitization quantum yields. Surprisingly, the ability of the complex to photo-inactivate bacteria of the strain Staphylococcus aureus is strongly reduced in the presence of DMSO, despite the higher yield of photosensitization. The reasons for this apparently contradictory behavior are investigated, providing new insights about the use of carrier systems for hydrophobic photosensitizers.
Tuning the local solvent composition at a drug carrier surface: Effect of dimethyl sulfoxide/water mixture on the photofunctional properties of hypericin--lactoglobulin complexes / Delcanale, Pietro; Rodríguez-Amigo, Beatriz; Juárez-Jiménez, Jordi; Luque, F. Javier; Abbruzzetti, Stefania; Agut, Montserrat; Nonell, Santi; Viappiani, Cristiano. - In: JOURNAL OF MATERIALS CHEMISTRY. B. - ISSN 2050-750X. - 5(2017), pp. 1633-1641. [10.1039/C7TB00081B]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2820602
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