Nanoparticles toxicity is associated with cardiovascular diseases (CDs). Although epidemiological studies confirmed such link, they did not prove a direct causation on cardiac tissue. Normotensive and hypertensive (SHR) rats were exposed to titanium dioxide (TiO2) and diesel exhaust particles (DEP) from Euro3 and Euro4 derived-engines. We explored the interaction between NPs and in-vivo/in-vitro cardiac tissue and we determined the effect on electro-mechanical performance by evaluating genetic, morphological, functional and toxicological alterations. We observed a direct contamination of cardiac tissue by tracheally-instilled NPs, correlated with structural remodeling, ROS, DNA damaged, modulation of ECGs and arrhythmogenesis. Such conditions are further aggravated in normotensive animal acutely exposed to Euro4-DEP and in SHR repeatedly exposed to TiO2-NPs. Such NPs produce transient nanopores (≤50 nm), which causes membrane leakage and action potential reduction. Our proposed approaches show a direct involvement of NPs linked to CDs, by establishing a novel arrhythmogenic mechanism.

Nanoparticles exposure: In-vitro and in-vivo investigation to evaluate cardiovascu1ar risk factors in normal and failing cardiac tissue [Esposizione a nanoparticelle: Studi in-vitro ed in-vivo per valutare i fattori di rischio cardiovascolari in cuori normali e patologici] / Miragoli, Michele; Rossi, Stefano; Savi, Monia; Goldoni, Matteo; Pinelli, Silvana; Alinovi, Rossella; Galetti, Maricla; Stilli, Donatella; Macchi, Emilio; Zaniboni, Massimiliano; Mutti, Antonio. - In: GIORNALE ITALIANO DI MEDICINA DEL LAVORO ED ERGONOMIA. - ISSN 1592-7830. - 38:3(2016), pp. 146-150.

Nanoparticles exposure: In-vitro and in-vivo investigation to evaluate cardiovascu1ar risk factors in normal and failing cardiac tissue [Esposizione a nanoparticelle: Studi in-vitro ed in-vivo per valutare i fattori di rischio cardiovascolari in cuori normali e patologici]

MIRAGOLI, MICHELE
;
ROSSI, Stefano;SAVI, Monia;GOLDONI, Matteo;PINELLI, Silvana;ALINOVI, Rossella;GALETTI, Maricla;STILLI, Donatella;MACCHI, Emilio;ZANIBONI, Massimiliano;MUTTI, Antonio
2016

Abstract

Nanoparticles toxicity is associated with cardiovascular diseases (CDs). Although epidemiological studies confirmed such link, they did not prove a direct causation on cardiac tissue. Normotensive and hypertensive (SHR) rats were exposed to titanium dioxide (TiO2) and diesel exhaust particles (DEP) from Euro3 and Euro4 derived-engines. We explored the interaction between NPs and in-vivo/in-vitro cardiac tissue and we determined the effect on electro-mechanical performance by evaluating genetic, morphological, functional and toxicological alterations. We observed a direct contamination of cardiac tissue by tracheally-instilled NPs, correlated with structural remodeling, ROS, DNA damaged, modulation of ECGs and arrhythmogenesis. Such conditions are further aggravated in normotensive animal acutely exposed to Euro4-DEP and in SHR repeatedly exposed to TiO2-NPs. Such NPs produce transient nanopores (≤50 nm), which causes membrane leakage and action potential reduction. Our proposed approaches show a direct involvement of NPs linked to CDs, by establishing a novel arrhythmogenic mechanism.
Nanoparticles exposure: In-vitro and in-vivo investigation to evaluate cardiovascu1ar risk factors in normal and failing cardiac tissue [Esposizione a nanoparticelle: Studi in-vitro ed in-vivo per valutare i fattori di rischio cardiovascolari in cuori normali e patologici] / Miragoli, Michele; Rossi, Stefano; Savi, Monia; Goldoni, Matteo; Pinelli, Silvana; Alinovi, Rossella; Galetti, Maricla; Stilli, Donatella; Macchi, Emilio; Zaniboni, Massimiliano; Mutti, Antonio. - In: GIORNALE ITALIANO DI MEDICINA DEL LAVORO ED ERGONOMIA. - ISSN 1592-7830. - 38:3(2016), pp. 146-150.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2820190
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