Group C rotaviruses (RVC) are enteric pathogens of humans and animals. Whole-genome sequences are available only for few RVCs, leaving gaps in our knowledge about their genetic diversity. We determined the full-length genome sequence of two human RVCs (PR2593/ 2004 and PR713/2012), detected in Italy from hospital-based surveillance for rotavirus infection in 2004 and 2012. In the 11 RNA genomic segments, the two Italian RVCs segregated within separate intra-genotypic lineages showed variation ranging from 1.9% (VP6) to 15.9% (VP3) at the nucleotide level. Comprehensive analysis of human RVC sequences available in the databases allowed us to reveal the existence of at least two major genome configurations, defined as type I and type II. Human RVCs of type I were all associated with the M3 VP3 genotype, including the Italian strain PR2593/2004. Conversely, human RVCs of type II were all associated with the M2 VP3 genotype, including the Italian strain PR713/2012. Reassortant RVC strains between these major genome configurations were identified. Although only a few full-genome sequences of human RVCs, mostly of Asian origin, are available, the analysis of human RVC sequences retrieved from the databases indicates that at least two intra-genotypic RVC lineages circulate in European countries. Gathering more sequence data is necessary to develop a standardized genotype and intragenotypic lineage classification system useful for epidemiological investigations and avoiding confusion in the literature.

Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment / Medici, Maria Cristina; Tummolo, Fabio; Martella, Vito; Arcangeletti, Maria Cristina; DE CONTO, Flora; Chezzi, Carlo; Fehér, Enikő; Marton, Szilvia; Calderaro, Adriana; Bányai, Krisztián. - In: JOURNAL OF GENERAL VIROLOGY. - ISSN 0022-1317. - 97:8(2016), pp. 1888-1898. [10.1099/jgv.0.000497]

Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment

MEDICI, Maria Cristina;TUMMOLO, FABIO;MARTELLA, VITO;ARCANGELETTI, Maria Cristina;DE CONTO, Flora;CHEZZI, Carlo;CALDERARO, Adriana;
2016

Abstract

Group C rotaviruses (RVC) are enteric pathogens of humans and animals. Whole-genome sequences are available only for few RVCs, leaving gaps in our knowledge about their genetic diversity. We determined the full-length genome sequence of two human RVCs (PR2593/ 2004 and PR713/2012), detected in Italy from hospital-based surveillance for rotavirus infection in 2004 and 2012. In the 11 RNA genomic segments, the two Italian RVCs segregated within separate intra-genotypic lineages showed variation ranging from 1.9% (VP6) to 15.9% (VP3) at the nucleotide level. Comprehensive analysis of human RVC sequences available in the databases allowed us to reveal the existence of at least two major genome configurations, defined as type I and type II. Human RVCs of type I were all associated with the M3 VP3 genotype, including the Italian strain PR2593/2004. Conversely, human RVCs of type II were all associated with the M2 VP3 genotype, including the Italian strain PR713/2012. Reassortant RVC strains between these major genome configurations were identified. Although only a few full-genome sequences of human RVCs, mostly of Asian origin, are available, the analysis of human RVC sequences retrieved from the databases indicates that at least two intra-genotypic RVC lineages circulate in European countries. Gathering more sequence data is necessary to develop a standardized genotype and intragenotypic lineage classification system useful for epidemiological investigations and avoiding confusion in the literature.
Analysis of the full genome of human group C rotaviruses reveals lineage diversification and reassortment / Medici, Maria Cristina; Tummolo, Fabio; Martella, Vito; Arcangeletti, Maria Cristina; DE CONTO, Flora; Chezzi, Carlo; Fehér, Enikő; Marton, Szilvia; Calderaro, Adriana; Bányai, Krisztián. - In: JOURNAL OF GENERAL VIROLOGY. - ISSN 0022-1317. - 97:8(2016), pp. 1888-1898. [10.1099/jgv.0.000497]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2818887
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