Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle-cell interactions. © 2014 American Chemical Society.
Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy / Novak, Pavel; Shevchuk, Andrew; Ruenraroengsak, Pakatip; Miragoli, Michele; Thorley, Andrew J.; Klenerman, David; Lab, Max J.; Tetley, Teresa D.; Gorelik, Julia; Korchev, Yuri E.. - In: NANO LETTERS. - ISSN 1530-6984. - 14:3(2014), pp. 1202-1207. [10.1021/nl404068p]
Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy
MIRAGOLI, MICHELE;
2014-01-01
Abstract
Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle-cell interactions. © 2014 American Chemical Society.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
