Ulcerative colitis (UC) is associated with a substantial alteration of specific gut commensals, some of which may be involved in microbiota-mediated protection. In this study, microbiota cataloging of UC patients by 16S rRNA microbial profiling revealed a marked reduction of bifidobacteria, in particular the Bifidobacterium bifidum species, thus suggesting that this taxon plays a biological role in the aetiology of UC. We investigated this further through an in vivo trial by testing the effects of oral treatment with B. bifidum PRL2010 in a wild-type murine colitis model. TNBS-treated mice receiving 109 cells of B. bifidum PRL2010 showed a marked reduction of all colitis-associated histological indices as well as maintenance of mucosal integrity as it was shown by the increase in the expression of many tight junction-encoding genes. The protective role of B. bifidum PRL2010, as well as its sortase-dependent pili, appears to be established through the induction of an innate immune response of the host. These results highlight the importance of B. bifidum as a microbial biomarker for UC, revealing its role in protection against experimentally induced colitis.

Elucidating the gut microbiome of ulcerative colitis: bifidobacteria as novel microbial biomarkers / Duranti, Sabrina; Gaiani, Federica; Mancabelli, Leonardo; Milani, Christian; Grandi, Andrea; Bolchi, Angelo; Santoni, Andrea; Lugli, Gabriele Andrea; Ferrario, Chiara; Mangifesta, Marta; Viappiani, Alice; Bertoni, Simona; Vivo, Valentina; Serafini, Fausta; Barbaro, Maria Raffaella; Fugazza, Alessandro; Barbara, Giovanni; Gioiosa, Laura; Palanza, Paola; Cantoni, Anna Maria; DE ANGELIS, Gian Luigi; Barocelli, Elisabetta; De’Angelis, Nicola; Sinderen, Douwe van; Ventura, Marco; Turroni, Francesca. - In: FEMS MICROBIOLOGY ECOLOGY. - ISSN 1574-6941. - 92:12(2016). [10.1093/femsec/fiw191]

Elucidating the gut microbiome of ulcerative colitis: bifidobacteria as novel microbial biomarkers

DURANTI, Sabrina;GAIANI, FEDERICA;MANCABELLI, Leonardo;MILANI, CHRISTIAN;GRANDI, Andrea;BOLCHI, Angelo;SANTONI, Andrea;LUGLI, Gabriele Andrea;FERRARIO, CHIARA;BERTONI, Simona;VIVO, Valentina;SERAFINI, Fausta;FUGAZZA, ALESSANDRO;GIOIOSA, Laura;PALANZA, Paola;CANTONI, Anna Maria;DE ANGELIS, Gian Luigi;BAROCELLI, Elisabetta;VENTURA, Marco;TURRONI, FRANCESCA
2016

Abstract

Ulcerative colitis (UC) is associated with a substantial alteration of specific gut commensals, some of which may be involved in microbiota-mediated protection. In this study, microbiota cataloging of UC patients by 16S rRNA microbial profiling revealed a marked reduction of bifidobacteria, in particular the Bifidobacterium bifidum species, thus suggesting that this taxon plays a biological role in the aetiology of UC. We investigated this further through an in vivo trial by testing the effects of oral treatment with B. bifidum PRL2010 in a wild-type murine colitis model. TNBS-treated mice receiving 109 cells of B. bifidum PRL2010 showed a marked reduction of all colitis-associated histological indices as well as maintenance of mucosal integrity as it was shown by the increase in the expression of many tight junction-encoding genes. The protective role of B. bifidum PRL2010, as well as its sortase-dependent pili, appears to be established through the induction of an innate immune response of the host. These results highlight the importance of B. bifidum as a microbial biomarker for UC, revealing its role in protection against experimentally induced colitis.
Elucidating the gut microbiome of ulcerative colitis: bifidobacteria as novel microbial biomarkers / Duranti, Sabrina; Gaiani, Federica; Mancabelli, Leonardo; Milani, Christian; Grandi, Andrea; Bolchi, Angelo; Santoni, Andrea; Lugli, Gabriele Andrea; Ferrario, Chiara; Mangifesta, Marta; Viappiani, Alice; Bertoni, Simona; Vivo, Valentina; Serafini, Fausta; Barbaro, Maria Raffaella; Fugazza, Alessandro; Barbara, Giovanni; Gioiosa, Laura; Palanza, Paola; Cantoni, Anna Maria; DE ANGELIS, Gian Luigi; Barocelli, Elisabetta; De’Angelis, Nicola; Sinderen, Douwe van; Ventura, Marco; Turroni, Francesca. - In: FEMS MICROBIOLOGY ECOLOGY. - ISSN 1574-6941. - 92:12(2016). [10.1093/femsec/fiw191]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2817584
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 85
  • ???jsp.display-item.citation.isi??? 86
social impact