Ulcerative colitis (UC) is associated with a substantial alteration of specific gut commensals, some of which may be involved in microbiota-mediated protection. In this study, microbiota cataloging of UC patients by 16S rRNA microbial profiling revealed a marked reduction of bifidobacteria, in particular the Bifidobacterium bifidum species, thus suggesting that this taxon plays a biological role in the aetiology of UC. We investigated this further through an in vivo trial by testing the effects of oral treatment with B. bifidum PRL2010 in a wild-type murine colitis model. TNBS-treated mice receiving 109 cells of B. bifidum PRL2010 showed a marked reduction of all colitis-associated histological indices as well as maintenance of mucosal integrity as it was shown by the increase in the expression of many tight junction-encoding genes. The protective role of B. bifidum PRL2010, as well as its sortase-dependent pili, appears to be established through the induction of an innate immune response of the host. These results highlight the importance of B. bifidum as a microbial biomarker for UC, revealing its role in protection against experimentally induced colitis.
Elucidating the gut microbiome of ulcerative colitis: bifidobacteria as novel microbial biomarkers / Duranti, S., Gaiani, F., Mancabelli, L., Milani, C., Grandi, A., Bolchi, A., Santoni, A., Lugli, G.A., Ferrario, C., Mangifesta, M., Viappiani, A., Bertoni, S., Vivo, V., Serafini, F., Barbaro, M.R., Fugazza, A., Barbara, G., Gioiosa, L., Palanza, P., Cantoni, A.M., et al.. - In: FEMS MICROBIOLOGY ECOLOGY. - ISSN 1574-6941. - 92:12(2016). [10.1093/femsec/fiw191]
Elucidating the gut microbiome of ulcerative colitis: bifidobacteria as novel microbial biomarkers
DURANTI, Sabrina;GAIANI, FEDERICA;MANCABELLI, Leonardo;MILANI, CHRISTIAN;GRANDI, Andrea;BOLCHI, Angelo;SANTONI, Andrea;LUGLI, Gabriele Andrea;FERRARIO, CHIARA;BERTONI, Simona;VIVO, Valentina;SERAFINI, Fausta;FUGAZZA, ALESSANDRO;GIOIOSA, Laura;PALANZA, Paola;CANTONI, Anna Maria;DE ANGELIS, Gian Luigi;BAROCELLI, Elisabetta;VENTURA, Marco;TURRONI, FRANCESCA
2016-01-01
Abstract
Ulcerative colitis (UC) is associated with a substantial alteration of specific gut commensals, some of which may be involved in microbiota-mediated protection. In this study, microbiota cataloging of UC patients by 16S rRNA microbial profiling revealed a marked reduction of bifidobacteria, in particular the Bifidobacterium bifidum species, thus suggesting that this taxon plays a biological role in the aetiology of UC. We investigated this further through an in vivo trial by testing the effects of oral treatment with B. bifidum PRL2010 in a wild-type murine colitis model. TNBS-treated mice receiving 109 cells of B. bifidum PRL2010 showed a marked reduction of all colitis-associated histological indices as well as maintenance of mucosal integrity as it was shown by the increase in the expression of many tight junction-encoding genes. The protective role of B. bifidum PRL2010, as well as its sortase-dependent pili, appears to be established through the induction of an innate immune response of the host. These results highlight the importance of B. bifidum as a microbial biomarker for UC, revealing its role in protection against experimentally induced colitis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
