Systemic inflammation participates to the complex healing process occurring after major surgery, thus directly affecting the surgical outcome and patient recovery. Total plasma N-glycome might be an indicator of inflammation after major surgery, as well as an anti-inflammatory therapy response marker, since protein glycosylation plays an essential role in the inflammatory cascade. Therefore, we assessed the effects of surgery on the total plasma N-glycome and the association with self-administration of postoperative morphine in two cohorts of patients that underwent major abdominal surgery. We found that plasma N-glycome undergoes significant changes one day after surgery and intensifies one day later, thus indicating a systemic physiological response. In particular, we observed the increase of bisialylated biantennary glycan, A2G2S[3,6]2, 12 hours after surgery, which progressively increased until 48 postoperative hours. Most changes occurred 24 hours after surgery with the decrease of most core-fucosylated biantennary structures, as well as the increase in sialylated tetraantennary and FA3G3S[3,3,3]3 structures. Moreover, we observed a progressive increase of sialylated triantennary and tetraantennary structures two days after surgery, with a concomitant decrease of the structures containing bisecting N-acetylglucosamine along with bi- and trisialylated triantennary glycans. We did not find any statistically significant association between morphine consumption and plasma N-glycome.

Changes in total plasma and serum N-glycome composition and patient-controlled analgesia after major abdominal surgery / Gudelj, Ivan; Baciarello, Marco; Ugrina, Ivo; De Gregori, Manuela; Napolioni, Valerio; Ingelmo, Pablo M.; Bugada, Dario; De Gregori, Simona; Derek, Lovorka; Pučić Baković, Maja; Novokmet, Mislav; Gornik, Olga; Saccani, Gloria; Meschi, Tiziana; Lauc, Gordan; Allegri, Massimo. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 6:(2016), p. 31234. [10.1038/srep31234]

Changes in total plasma and serum N-glycome composition and patient-controlled analgesia after major abdominal surgery

BACIARELLO, Marco;BUGADA, DARIO;SACCANI, Gloria;MESCHI, Tiziana;ALLEGRI, Massimo
2016-01-01

Abstract

Systemic inflammation participates to the complex healing process occurring after major surgery, thus directly affecting the surgical outcome and patient recovery. Total plasma N-glycome might be an indicator of inflammation after major surgery, as well as an anti-inflammatory therapy response marker, since protein glycosylation plays an essential role in the inflammatory cascade. Therefore, we assessed the effects of surgery on the total plasma N-glycome and the association with self-administration of postoperative morphine in two cohorts of patients that underwent major abdominal surgery. We found that plasma N-glycome undergoes significant changes one day after surgery and intensifies one day later, thus indicating a systemic physiological response. In particular, we observed the increase of bisialylated biantennary glycan, A2G2S[3,6]2, 12 hours after surgery, which progressively increased until 48 postoperative hours. Most changes occurred 24 hours after surgery with the decrease of most core-fucosylated biantennary structures, as well as the increase in sialylated tetraantennary and FA3G3S[3,3,3]3 structures. Moreover, we observed a progressive increase of sialylated triantennary and tetraantennary structures two days after surgery, with a concomitant decrease of the structures containing bisecting N-acetylglucosamine along with bi- and trisialylated triantennary glycans. We did not find any statistically significant association between morphine consumption and plasma N-glycome.
Changes in total plasma and serum N-glycome composition and patient-controlled analgesia after major abdominal surgery / Gudelj, Ivan; Baciarello, Marco; Ugrina, Ivo; De Gregori, Manuela; Napolioni, Valerio; Ingelmo, Pablo M.; Bugada, Dario; De Gregori, Simona; Derek, Lovorka; Pučić Baković, Maja; Novokmet, Mislav; Gornik, Olga; Saccani, Gloria; Meschi, Tiziana; Lauc, Gordan; Allegri, Massimo. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 6:(2016), p. 31234. [10.1038/srep31234]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2812633
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