This study evaluated the immunochromatographic (IC) assay "TECHLAB® E. HISTOLYTICA QUIK CHEK™" analysing 36 faecal samples and 7 cultured strains. This assay was compared to the methods performed in our laboratory for the diagnosis of amoebiasis. The IC assay revealed a detection limit of 103 trophozoites/g faeces and no cross-reactivity with other parasites and failed to detect E. histolytica antigen in frozen faeces. In our laboratory located in a non-endemic setting this assay could not replace the methods currently used for the diagnosis of amoebiasis.

Diagnostic performances of antigen detection compared to conventional and nucleic acid detection of Entamoeba histolytica in a non-endemic setting / Calderaro, Adriana; Piergianni, Maddalena; Piccolo, Giovanna; Rossi, Sabina; Montecchini, Sara; Buttrini, Mirko; Arcangeletti, Maria Cristina; Medici, Maria Cristina; Chezzi, Carlo; DE CONTO, Flora. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 39:2(2016), pp. 153-155.

Diagnostic performances of antigen detection compared to conventional and nucleic acid detection of Entamoeba histolytica in a non-endemic setting

CALDERARO, Adriana;PIERGIANNI, Maddalena;PICCOLO, Giovanna;ROSSI, Sabina;MONTECCHINI, Sara;BUTTRINI, Mirko;ARCANGELETTI, Maria Cristina;MEDICI, Maria Cristina;CHEZZI, Carlo;DE CONTO, Flora
2016-01-01

Abstract

This study evaluated the immunochromatographic (IC) assay "TECHLAB® E. HISTOLYTICA QUIK CHEK™" analysing 36 faecal samples and 7 cultured strains. This assay was compared to the methods performed in our laboratory for the diagnosis of amoebiasis. The IC assay revealed a detection limit of 103 trophozoites/g faeces and no cross-reactivity with other parasites and failed to detect E. histolytica antigen in frozen faeces. In our laboratory located in a non-endemic setting this assay could not replace the methods currently used for the diagnosis of amoebiasis.
Diagnostic performances of antigen detection compared to conventional and nucleic acid detection of Entamoeba histolytica in a non-endemic setting / Calderaro, Adriana; Piergianni, Maddalena; Piccolo, Giovanna; Rossi, Sabina; Montecchini, Sara; Buttrini, Mirko; Arcangeletti, Maria Cristina; Medici, Maria Cristina; Chezzi, Carlo; DE CONTO, Flora. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 39:2(2016), pp. 153-155.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2808394
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