The biological activity of a combined treatment of U251, U373 and T98G glioma cell lines with two anti-miR PNAs, directed against miR-221 and miR-222 and conjugated with an ocataarginine tail (R8-PNA-a221 and R8-PNA-a222) for efficient cellular delivery, was determined. Apoptosis was analyzed, and the effect of the combined treatment of glioma cells with either or both PNAs on the reversion of drug-resistance phenotype was assessed in the temozolomideresistant T98G glioma cell line. Selectivity of PNA/miRNA interactions was studied by surface plasmon resonance (SPR)- based Biacore analysis. Specificity of the PNA effects at the cellular level was analyzed by RT-qPCR. These experiments support the concept that the effects of R8-PNA-a221 and R8-PNA-a222 are specific. The studies on apoptosis confirmed that the R8-PNA-a221 induces apoptosis and demonstrated the pro-apoptotic effects of R8-PNA-a222. Remarkably, increased pro-apoptotic effects were obtained with the co-administration of both anti-miR-221 and anti-miR-222 PNAs. In addition, co-administration of R8-PNA-a221 and R8-PNA-a222 induced apoptosis of TMZ-treated T98G cells at a level higher than that obtained following singular administration of R8-PNA-a221 or R8-PNA-a222.

High levels of apoptosis are induced in human glioma cell lines by co-administration of peptide nucleic acids targeting miR-221 and miR-222 / Brognara, Eleonora; Fabbri, Enrica; Montagner, Giulia; Gasparello, Jessica; Manicardi, Alex; Corradini, Roberto; Bianchi, Nicoletta; Finotti, Alessia; Breveglieri, Giulia; Borgatti, Monica; Lampronti, Ilaria; Milani, Roberta; Dechecchi, Maria Cristina; Cabrini, Giulio; Gambari, Roberto. - In: INTERNATIONAL JOURNAL OF ONCOLOGY. - ISSN 1019-6439. - 48:3(2016), pp. 1029-1038. [10.3892/ijo.2015.3308]

High levels of apoptosis are induced in human glioma cell lines by co-administration of peptide nucleic acids targeting miR-221 and miR-222

MANICARDI, Alex;CORRADINI, Roberto;
2016

Abstract

The biological activity of a combined treatment of U251, U373 and T98G glioma cell lines with two anti-miR PNAs, directed against miR-221 and miR-222 and conjugated with an ocataarginine tail (R8-PNA-a221 and R8-PNA-a222) for efficient cellular delivery, was determined. Apoptosis was analyzed, and the effect of the combined treatment of glioma cells with either or both PNAs on the reversion of drug-resistance phenotype was assessed in the temozolomideresistant T98G glioma cell line. Selectivity of PNA/miRNA interactions was studied by surface plasmon resonance (SPR)- based Biacore analysis. Specificity of the PNA effects at the cellular level was analyzed by RT-qPCR. These experiments support the concept that the effects of R8-PNA-a221 and R8-PNA-a222 are specific. The studies on apoptosis confirmed that the R8-PNA-a221 induces apoptosis and demonstrated the pro-apoptotic effects of R8-PNA-a222. Remarkably, increased pro-apoptotic effects were obtained with the co-administration of both anti-miR-221 and anti-miR-222 PNAs. In addition, co-administration of R8-PNA-a221 and R8-PNA-a222 induced apoptosis of TMZ-treated T98G cells at a level higher than that obtained following singular administration of R8-PNA-a221 or R8-PNA-a222.
High levels of apoptosis are induced in human glioma cell lines by co-administration of peptide nucleic acids targeting miR-221 and miR-222 / Brognara, Eleonora; Fabbri, Enrica; Montagner, Giulia; Gasparello, Jessica; Manicardi, Alex; Corradini, Roberto; Bianchi, Nicoletta; Finotti, Alessia; Breveglieri, Giulia; Borgatti, Monica; Lampronti, Ilaria; Milani, Roberta; Dechecchi, Maria Cristina; Cabrini, Giulio; Gambari, Roberto. - In: INTERNATIONAL JOURNAL OF ONCOLOGY. - ISSN 1019-6439. - 48:3(2016), pp. 1029-1038. [10.3892/ijo.2015.3308]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2808282
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