We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy. After introducing furan-containing building blocks into a PNA sequence, hybridization and furan-oxidation based crosslinking to DNA is investigated. The structure of the crosslinked products is characterized and preliminary investigations concerning the application of these systems to double stranded DNA are shown.
Furan-PNA: A mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA / Manicardi, Alex; Gyssels, E.; Corradini, Roberto; Madder, A.. - In: CHEMICAL COMMUNICATIONS. - ISSN 1359-7345. - 52:42(2016), pp. 6930-6933. [10.1039/c6cc02062c]
Furan-PNA: A mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA
MANICARDI, Alex;CORRADINI, Roberto;
2016-01-01
Abstract
We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy. After introducing furan-containing building blocks into a PNA sequence, hybridization and furan-oxidation based crosslinking to DNA is investigated. The structure of the crosslinked products is characterized and preliminary investigations concerning the application of these systems to double stranded DNA are shown.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
