Inflammation plays an important role in the pathogenesis of congestive heart failure (CHF). In humans with CHF, increased production and high plasma concentrations of tumour necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1, IL-8 and transforming growth factor-β (TGF-β) have been associated with disease progression and a negative prognosis. The aim of this study was to investigate whether differences in cytokine blood mRNA expression exist between clinically healthy dogs and dogs with myxomatous mitral valve disease (MMVD); to determine if the expression was related to the severity of MMVD, and to detect any correlations with echocardiographic parameters of cardiac remodelling. Twenty-three dogs with MMVD of varying severity and six clinically healthy dogs were included in the study. Whole blood samples were obtained for measurement of mRNA expression of IL-1α, IL-1β, IL-6, IL-8, TGF-β1, TNF-α by reverse transcriptase-PCR (RT-PCR). There were statistically significant differences between clinically healthy dogs and dogs with MMVD for IL-8 and TGF-β1 gene expression. IL-8 expression increased with increasing MMVD severity and TGF-β1 expression was higher in asymptomatic dogs with echocardiographic signs of cardiac remodelling (American College Veterinary Internal Medicine class B2) than in all other groups. These results could suggest the involvement of these cytokines at different stages of the disease.

Cytokine expression in peripheral blood mononuclear cells of dogs with mitral valve disease / Mavropoulou, A; Guazzetti, S; Borghetti, Paolo; De Angelis, Elena; Quintavalla, Cecilia. - In: THE VETERINARY JOURNAL. - ISSN 1090-0233. - 211:1(2016), pp. 45-51. [10.1016/j.tvjl.2016.03.002]

Cytokine expression in peripheral blood mononuclear cells of dogs with mitral valve disease

BORGHETTI, Paolo;DE ANGELIS, Elena;QUINTAVALLA, Cecilia
2016

Abstract

Inflammation plays an important role in the pathogenesis of congestive heart failure (CHF). In humans with CHF, increased production and high plasma concentrations of tumour necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1, IL-8 and transforming growth factor-β (TGF-β) have been associated with disease progression and a negative prognosis. The aim of this study was to investigate whether differences in cytokine blood mRNA expression exist between clinically healthy dogs and dogs with myxomatous mitral valve disease (MMVD); to determine if the expression was related to the severity of MMVD, and to detect any correlations with echocardiographic parameters of cardiac remodelling. Twenty-three dogs with MMVD of varying severity and six clinically healthy dogs were included in the study. Whole blood samples were obtained for measurement of mRNA expression of IL-1α, IL-1β, IL-6, IL-8, TGF-β1, TNF-α by reverse transcriptase-PCR (RT-PCR). There were statistically significant differences between clinically healthy dogs and dogs with MMVD for IL-8 and TGF-β1 gene expression. IL-8 expression increased with increasing MMVD severity and TGF-β1 expression was higher in asymptomatic dogs with echocardiographic signs of cardiac remodelling (American College Veterinary Internal Medicine class B2) than in all other groups. These results could suggest the involvement of these cytokines at different stages of the disease.
Cytokine expression in peripheral blood mononuclear cells of dogs with mitral valve disease / Mavropoulou, A; Guazzetti, S; Borghetti, Paolo; De Angelis, Elena; Quintavalla, Cecilia. - In: THE VETERINARY JOURNAL. - ISSN 1090-0233. - 211:1(2016), pp. 45-51. [10.1016/j.tvjl.2016.03.002]
File in questo prodotto:
File Dimensione Formato  
cytokines.pdf

accesso aperto

Descrizione: Articolo principale
Tipologia: Documento in Post-print
Licenza: Creative commons
Dimensione 382.13 kB
Formato Adobe PDF
382.13 kB Adobe PDF Visualizza/Apri
figure 1.jpg

accesso aperto

Descrizione: Figura
Tipologia: Documento in Post-print
Licenza: Creative commons
Dimensione 32.25 kB
Formato JPEG
32.25 kB JPEG Visualizza/Apri
figure 2.jpg

accesso aperto

Descrizione: Figura
Tipologia: Documento in Post-print
Licenza: Creative commons
Dimensione 58.98 kB
Formato JPEG
58.98 kB JPEG Visualizza/Apri
figure 3.jpg

accesso aperto

Descrizione: Figura
Tipologia: Documento in Post-print
Licenza: Creative commons
Dimensione 35 kB
Formato JPEG
35 kB JPEG Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2806497
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 7
social impact