Glycoligands, sugar-based molecules able to complex metal cations, constitute a new class of molecules with great potential for biological and biochemical applications. To analyze their behaviour in a biological environment, we have synthesized an intrinsically fluorescent glycoligand and analyzed its trafficking in both living (U937 human cancer cells) and artificial (giant unilamellar vesicles) cell systems. We have found that this ligand has moderate cytotoxicity accompanied by specific accumulation in both living and reconstituted membranes, which it can cross to reach inner compartments.

An intrinsically fluorescent glycoligand for direct imaging of ligand trafficking in artificial and living cell systems / Ludivine Garcia;Mirca Lazzaretti;Antoine Diguet;Francesca Mussi;Franco Bisceglie;Juan Xie;Giorgio Pelosi;Annamaria Buschini;Damien Baigl;Clotilde Policar. - In: NEW JOURNAL OF CHEMISTRY. - ISSN 1144-0546. - 37:10(2013), pp. 3030-3034. [10.1039/c3nj00380a]

An intrinsically fluorescent glycoligand for direct imaging of ligand trafficking in artificial and living cell systems

MUSSI, FRANCESCA;BISCEGLIE, Franco;PELOSI, Giorgio;BUSCHINI, Annamaria;
2013

Abstract

Glycoligands, sugar-based molecules able to complex metal cations, constitute a new class of molecules with great potential for biological and biochemical applications. To analyze their behaviour in a biological environment, we have synthesized an intrinsically fluorescent glycoligand and analyzed its trafficking in both living (U937 human cancer cells) and artificial (giant unilamellar vesicles) cell systems. We have found that this ligand has moderate cytotoxicity accompanied by specific accumulation in both living and reconstituted membranes, which it can cross to reach inner compartments.
An intrinsically fluorescent glycoligand for direct imaging of ligand trafficking in artificial and living cell systems / Ludivine Garcia;Mirca Lazzaretti;Antoine Diguet;Francesca Mussi;Franco Bisceglie;Juan Xie;Giorgio Pelosi;Annamaria Buschini;Damien Baigl;Clotilde Policar. - In: NEW JOURNAL OF CHEMISTRY. - ISSN 1144-0546. - 37:10(2013), pp. 3030-3034. [10.1039/c3nj00380a]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2651264
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