We describe a novel sensing approach based on a functionalized microstructured optical fiber-Bragg grating for specific DNA target sequences detection. The inner surface of a microstructured fiber, where a Bragg grating was previously inscribed, has been functionalized by covalent linking of a peptide nucleic acid probe targeting a DNA sequence bearing a single point mutation implicated in cystic fibrosis (CF) disease. A solution of an oligonucleotide (ON) corresponding to a tract of the CF gene containing the mutated DNA has been infiltrated inside the fiber capillaries and allowed to hybridize to the fiber surface according to the Watson-Crick pairing. In order to achieve signal amplification, ON-functionalized gold nanoparticles were then infiltrated and used in a sandwich-like assay. Experimental measurements show a clear shift of the reflected high order mode of a Bragg grating for a 100 nM DNA solution, and fluorescence measurements have confirmed the successful hybridization. Several experiments have been carried out on the same fiber using the identical concentration, showing the same modulation trend, suggesting the possibility of the reuse of the sensor. Measurements have also been made using a 100 nM mismatched DNA solution, containing a single nucleotide mutation and corresponding to the wild-type gene, and the results demonstrate the high selectivity of the sensor.

Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating / A. Candiani; A. Bertucci; S. Giannetti; M. Konstantaki; A. Manicardi; S. Pissadakis; A. Cucinotta; R. Corradini; S. Selleri. - In: JOURNAL OF BIOMEDICAL OPTICS. - ISSN 1083-3668. - 18:5(2013), pp. 057004-1-057004-6. [10.1117/1.JBO.18.5.057004]

Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating

CANDIANI, Alessandro;BERTUCCI, Alessandro;MANICARDI, Alex;CUCINOTTA, Annamaria;CORRADINI, Roberto;SELLERI, Stefano
2013

Abstract

We describe a novel sensing approach based on a functionalized microstructured optical fiber-Bragg grating for specific DNA target sequences detection. The inner surface of a microstructured fiber, where a Bragg grating was previously inscribed, has been functionalized by covalent linking of a peptide nucleic acid probe targeting a DNA sequence bearing a single point mutation implicated in cystic fibrosis (CF) disease. A solution of an oligonucleotide (ON) corresponding to a tract of the CF gene containing the mutated DNA has been infiltrated inside the fiber capillaries and allowed to hybridize to the fiber surface according to the Watson-Crick pairing. In order to achieve signal amplification, ON-functionalized gold nanoparticles were then infiltrated and used in a sandwich-like assay. Experimental measurements show a clear shift of the reflected high order mode of a Bragg grating for a 100 nM DNA solution, and fluorescence measurements have confirmed the successful hybridization. Several experiments have been carried out on the same fiber using the identical concentration, showing the same modulation trend, suggesting the possibility of the reuse of the sensor. Measurements have also been made using a 100 nM mismatched DNA solution, containing a single nucleotide mutation and corresponding to the wild-type gene, and the results demonstrate the high selectivity of the sensor.
Label-free DNA biosensor based on a peptide nucleic acid-functionalized microstructured optical fiber-Bragg grating / A. Candiani; A. Bertucci; S. Giannetti; M. Konstantaki; A. Manicardi; S. Pissadakis; A. Cucinotta; R. Corradini; S. Selleri. - In: JOURNAL OF BIOMEDICAL OPTICS. - ISSN 1083-3668. - 18:5(2013), pp. 057004-1-057004-6. [10.1117/1.JBO.18.5.057004]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2616248
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