The increased incidence and severity of Clostridium difficile infection, particularly in North America and Europe, have brought renewed focus on the most appropriate method to detect C. difficile and/or its toxins in stools. This prospective study evaluated the usefulness of the Illumigene TM C. difficile assay in diagnostic practice for the detection of toxigenic C. difficile DNA in clinical samples. A total of 88 out of 306 stool samples analysed were positive both by Illumigene and the combination of toxigenic C. difficile culture (TC) and immunochromatographic assay (IC) with a concordance of 100%. Of the 218 samples negative by the combination of TC and IC, 204 were negative also by Illumigene with a concordance of 93.57%. In our experience, compared to conventional assays Illumigene assay proved to be easy to perform, accurate and prompt giving results within 1 hour at a cost of 28 euro per sample

Comparative analysis of different methods to detect Clostridium difficile infection / Calderaro, Adriana; Buttrini, Mirko; Martinelli, Monica; Gorrini, Chiara; Montecchini, Sara; Medici, Maria Cristina; Arcangeletti, Maria Cristina; DE CONTO, Flora; Covan, Silvia; Chezzi, Carlo. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 36:1(2013), pp. 57-63.

Comparative analysis of different methods to detect Clostridium difficile infection

CALDERARO, Adriana;BUTTRINI, Mirko;MARTINELLI, Monica;GORRINI, Chiara;MONTECCHINI, Sara;MEDICI, Maria Cristina;ARCANGELETTI, Maria Cristina;DE CONTO, Flora;COVAN, Silvia;CHEZZI, Carlo
2013-01-01

Abstract

The increased incidence and severity of Clostridium difficile infection, particularly in North America and Europe, have brought renewed focus on the most appropriate method to detect C. difficile and/or its toxins in stools. This prospective study evaluated the usefulness of the Illumigene TM C. difficile assay in diagnostic practice for the detection of toxigenic C. difficile DNA in clinical samples. A total of 88 out of 306 stool samples analysed were positive both by Illumigene and the combination of toxigenic C. difficile culture (TC) and immunochromatographic assay (IC) with a concordance of 100%. Of the 218 samples negative by the combination of TC and IC, 204 were negative also by Illumigene with a concordance of 93.57%. In our experience, compared to conventional assays Illumigene assay proved to be easy to perform, accurate and prompt giving results within 1 hour at a cost of 28 euro per sample
Comparative analysis of different methods to detect Clostridium difficile infection / Calderaro, Adriana; Buttrini, Mirko; Martinelli, Monica; Gorrini, Chiara; Montecchini, Sara; Medici, Maria Cristina; Arcangeletti, Maria Cristina; DE CONTO, Flora; Covan, Silvia; Chezzi, Carlo. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 36:1(2013), pp. 57-63.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2524687
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