We introduce a novel fluorescent reporter with potential for super-resolution microscopy, based on the bacterial photoreceptor YtvA. YtvA (from Bacillus subtilis) comprises a photosensitive flavin based LOV domain, efficiently photoswitchable between fluorescent and non-fluorescent states. We demonstrate Fluorescence PhotoActivation Localization Microscopy (FPALM) studies of live Escherichia coli cells, expressing YtvA molecules.
A photochromic bacterial photoreceptor with potential for super-resolution microscopy / Losi, Aba; W., Gärtner; S., Raffelberg; F., Cella Zanacchi; P., Bianchini; A., Diaspro; Mandalari, Carmen; Abbruzzetti, Stefania; Viappiani, Cristiano. - In: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES. - ISSN 1474-905X. - 12:(2013), pp. 231-235. [10.1039/c2pp25254f]
A photochromic bacterial photoreceptor with potential for super-resolution microscopy
LOSI, Aba;MANDALARI, Carmen;ABBRUZZETTI, Stefania;VIAPPIANI, Cristiano
2013-01-01
Abstract
We introduce a novel fluorescent reporter with potential for super-resolution microscopy, based on the bacterial photoreceptor YtvA. YtvA (from Bacillus subtilis) comprises a photosensitive flavin based LOV domain, efficiently photoswitchable between fluorescent and non-fluorescent states. We demonstrate Fluorescence PhotoActivation Localization Microscopy (FPALM) studies of live Escherichia coli cells, expressing YtvA molecules.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
