This study evaluated the absorption and metabolism of milk thistle flavonolignans silychristin, silydianin, silybin and isosilybin isomers (all together known as silymarin) in humans. Fourteen volunteers consumed an extract of milk thistle and urine was collected up to 48 hours after consumption. Thirty-one metabolites were identified in urine by means of HPLC-MS/MS, monoglucuronides being the most common excreted form, followed by sulphate-glucuronides and diglucuronides, respectively. The excretion of monoglucuronides peaked 2 hours after consumption, whereas sulphate-glucuronide and diglucuronide excretion peaked at 8 hours. The bioavailability of milk thistle flavonolignans was 0.45 ± 0.28% (mean ± SD). In conclusion, milk thistle flavonolignans are extensively modified after ingestion and recovered in urine as sulpho- and glucuronyl-conjugates, indicating a strong affinity for hepatic phase II enzymes. All future studies (in vitro and in vivo) dealing with the effects of milk thistle should start by considering the modification of its flavonolignans after ingestion by humans.

Absorption and metabolism of milk thistle flavonolignans in humans / L Calani; F Brighenti; R Bruni; D Del Rio. - In: PHYTOMEDICINE. - ISSN 0944-7113. - 20:1(2012), pp. 40-46. [10.1016/j.phymed.2012.09.004]

Absorption and metabolism of milk thistle flavonolignans in humans

CALANI, Luca;BRIGHENTI, Furio;BRUNI, Renato;DEL RIO, Daniele
2012

Abstract

This study evaluated the absorption and metabolism of milk thistle flavonolignans silychristin, silydianin, silybin and isosilybin isomers (all together known as silymarin) in humans. Fourteen volunteers consumed an extract of milk thistle and urine was collected up to 48 hours after consumption. Thirty-one metabolites were identified in urine by means of HPLC-MS/MS, monoglucuronides being the most common excreted form, followed by sulphate-glucuronides and diglucuronides, respectively. The excretion of monoglucuronides peaked 2 hours after consumption, whereas sulphate-glucuronide and diglucuronide excretion peaked at 8 hours. The bioavailability of milk thistle flavonolignans was 0.45 ± 0.28% (mean ± SD). In conclusion, milk thistle flavonolignans are extensively modified after ingestion and recovered in urine as sulpho- and glucuronyl-conjugates, indicating a strong affinity for hepatic phase II enzymes. All future studies (in vitro and in vivo) dealing with the effects of milk thistle should start by considering the modification of its flavonolignans after ingestion by humans.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11381/2450436
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