Abstract The multiplication of Ulster 73 virus, an avian strain of type A influenza virus, was blocked in chick embryo fibroblast cells, CEF, by treatment with 0.5 microg/ml of chromomycin A3 whereas in LLC-MK2 cells no inhibition of replication was observed. Virus-induced polypeptide synthesis in chick embryo fibroblast cells was confined to the synthesis of PB2, PB1 and PA subunits of the RNA dependent-RNA polymerase, the nucleoprotein NP, the non-structural protein NS1, the haemagglutinin HA, the non-structural protein NS2; only the membrane M1 polypeptide synthesis was greatly inhibited. Viral unpolyadenylated cRNAs synthesis was studied at a late time of the infection, 8 hours p.i.: chromomycin A3 was able to inhibit the "novo" synthesis of complementary RNA poly(A)- and segment 7 of virion RNA. The mode of action of the drug in chick embryo fibroblast cells is discussed. PMID: 12437217 [PubMed - indexed for MEDLINE]

Chromomycin A3 inhibits influenza a virus multiplication in chick embryo fibroblast cells / Conti, Giorgio; Portincasa, Pietro. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 25(4):(2002), pp. 385-398.

Chromomycin A3 inhibits influenza a virus multiplication in chick embryo fibroblast cells

CONTI, Giorgio;PORTINCASA, Pietro
2002-01-01

Abstract

Abstract The multiplication of Ulster 73 virus, an avian strain of type A influenza virus, was blocked in chick embryo fibroblast cells, CEF, by treatment with 0.5 microg/ml of chromomycin A3 whereas in LLC-MK2 cells no inhibition of replication was observed. Virus-induced polypeptide synthesis in chick embryo fibroblast cells was confined to the synthesis of PB2, PB1 and PA subunits of the RNA dependent-RNA polymerase, the nucleoprotein NP, the non-structural protein NS1, the haemagglutinin HA, the non-structural protein NS2; only the membrane M1 polypeptide synthesis was greatly inhibited. Viral unpolyadenylated cRNAs synthesis was studied at a late time of the infection, 8 hours p.i.: chromomycin A3 was able to inhibit the "novo" synthesis of complementary RNA poly(A)- and segment 7 of virion RNA. The mode of action of the drug in chick embryo fibroblast cells is discussed. PMID: 12437217 [PubMed - indexed for MEDLINE]
Chromomycin A3 inhibits influenza a virus multiplication in chick embryo fibroblast cells / Conti, Giorgio; Portincasa, Pietro. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 25(4):(2002), pp. 385-398.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11381/2438881
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