Migration of influenza virus-specific polypeptides from cytoplasm to nucleus of infected cells.

Abstract Previous studies have shown that various influenzavirus polypeptides are associated with the infected cell nucleus. To clarify the pattern of distribution of influenzavirus-specific polypeptides between nucleus and cytoplasm in infected cells and to gain further information concerning functions of influenzavirus replication occurring in the host cell nucleus, we have used high-resolution pulse-labeling experiments and detailed ultrastructural examination of purified preparations of nuclei. In addition to NP and NS1, both P1 and P2 associate preferentially with the nucleus, while P3 and NS2 remain in the cytoplasm. The viral M protein is seen in nuclear fractions only when these fractions are significantly contaminated by plasma membrane. The preferential association of P1 and P2 with nuclei suggests that these proteins, in conjunction with NP and NS1, are involved in specific functions which may include viral transcription, while P3 and NS2 are involved in a distinct role in the cytoplasm. Author to whom reprint requests should be sent.1 Present address: The Rockefeller University, 1230 York Avenue, New York, N. Y. 10021. 2 Permanent address: Istituto di Microbiologia, Universita Degli Studi di Parma, Italy. Copyright © 1981 Published by Elsevier Inc. 139 articles match your search: "g conti migration nuclear cytoplasmic influenza virus " Copyright © 2012 Elsevier B.V. All rights reserved. SciVerse® is a registered trademark of Elsevier Properties S.A., used under license. ScienceDirect® is a registered trademark of Elsevier B.V.