Mouse monoclonal antibodies against brush-border antigens of the proximal tubule of human kidney were produced by the hybridisation technique. The urinary excretion of a brush-border protein with an apparent molecular weight of 50 000 (BB-50) was measured by a sandwich enzyme-linked immunosorbent assay with a mouse IgG1 against BB-50 and a polyclonal rabbit antiserum against human kidney as coating and second antibodies. The urinary excretion of BB-50 was fifty times higher in patients treated with cisplatin than in a matched control group and twice as high in workers occupationally exposed to water-soluble chromium(VI) compounds as in their matched controls. These findings suggest that the urinary excretion of kidney antigens revealed by monoclonal antibodies is a very sensitive and specific test for the assessment of toxic nephropathies.
Urinary excretion of brush-border antigen revealed by monoclonal antibody: early indicator of toxic nephropathy / Mutti A; Lucertini S; Valcavi P; Neri TM; Fornari M; Alinovi R; Franchini I.. - In: THE LANCET. - ISSN 0140-6736. - 2(8461)(1985), pp. 914-917.
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